20th International Congress
on In Vitro Toxicology (ESTIV2018)

New approach methodologies for in vitro toxicology applications
15-18 October 2018 • Berlin / Germany

20th International Congress
on In Vitro Toxicology (ESTIV2018)

New approach methodologies for in vitro toxicology applications
15-18 October 2018 • Berlin / Germany

Congress details

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Scientific programme

The regular sessions of the scientific programme will be completed with submitted abstracts selected for oral presentations.

Preconference workshop

09.00–11.30
Room
Chairs
Preconference workshop 1 (ESTIV/JRC): AOPs: an essential tool for in vitro toxicology
Main auditorium
Maurice Whelan (Ispra/IT), Mathieu Vinken (Brussels/BE)
Panel members Mirjam Luijten (Utrecht/NL)
Brigitte Landesmann (Ispra/IT)
Dan Villeneuve (Duluth, MN/US)
Annamaria Carusi (Sheffield/UK)
11.30–13.30
Room
Chairs
Preconference workshop 2 (ESTIV/CAAT-EU): Good in vitro method practices/Good cell culture practice (GIVIMP/GCCP)
Main auditorium
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.30–11.40 Introduction
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.40–12.10 Overview of the OECD guidance document on GIVIMP
Sandra Coecke (Ispra/IT)
12.10–12.30 Fetal bovine serum-free media and serum alternatives
Jan van der Valk (Utrecht/NL)
12.30–12.50 GCCP – past, present and future
David Pamies (Baltimore/US)
12.50–13.10 GCCP and human pluripotent stem cell lines
Glyn Stacey (Hertfordshire/GB)
13.10–13.30 Discussion with public and panel
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)

Programme

09.00–11.30
Room
Chairs
Preconference workshop 1 (ESTIV/JRC): AOPs: an essential tool for in vitro toxicology
Main auditorium
Maurice Whelan (Ispra/IT), Mathieu Vinken (Brussels/BE)
Panel membersMirjam Luijten (Utrecht/NL)
Brigitte Landesmann (Ispra/IT)
Dan Villeneuve (Duluth, MN/US)
Annamaria Carusi (Sheffield/UK)
11.30–13.30
Room
Preconference workshop 2 (ESTIV/CAAT-EU): Good in vitro method practices/Good cell culture practice (GIVIMP/GCCP) 
Main auditorium
13.30–14.00
Room
Lunch, poster viewing and exhibition
Foyer
14.00–15.15
Room
Opening and keynote lecture
Main auditorium
Bioengineering the next generation of 3D cell models
Paula Alves (Oeiras/PT)
15.15–16.00
Room
Chair
Björn Ekwall award ceremony and lecture
Main auditorium
Stina Oredsson (Lund/SE)
 Mechanism-driven estimation of chemically induced eye nociception and irritation 
Anna Forsby (Stockholm/SE)
16.30–19.00
Room
Chairs
Session 1 – Bio-engineering and stem cell models
Main auditorium
Glyn Stacey (Hertfordshire/GB), João Barroso (Ispra/IT)
 Building qualified tissue using advanced in vitro methods
Glyn Stacey (Hertfordshire/GB)
Application of 3D liver microtissues for assessment of drug-induced liver Injury
Tomas Hejal (Schlieren/CH)
19.00–20.30
Room
Welcome reception
Industrial exhibition
08.30–10.30
Room
Chairs
Session 2 – Toxicokinetics and in vitro-in vivo extrapolation
Main auditorium
Nynke Kramer (Utrecht/NL), Paul Jennings (Amsterdam/NL)
 In vitro biokinetic considerations in quantitative in vitro-in vivo extrapolation studies
Nynke Kramer (Utrecht/NL)
Simulating foetal drug exposure: sildenafil as an example
Pieter Annaert (Leuven/NL)
11.00–12.30
Room
Chairs
Session 3 – Models, biomarkers and assays for systemic toxicity testing
Main auditorium
Aldert Piersma (Bilthoven/NL), Marcel Leist (Konstanz/DE)
11.00 Integrated approaches to testing and assessment in developmental toxicology
Aldert Piersma (Bilthoven/NL)
12.05 Rapid and accurate high-throughput in vitro prediction of nephrotoxicity in human with proxtox-hts
Daniele Zink (Singapore/SG)
12.30–13.30
Room
Lunch session – Updates from the Japanese Society for Alternatives to Animal Experiments (JSAAE)
Main auditorium

Japanese strategy on alternative to animal test methods for systemic toxicology
Hajime Kojima (Tokio/JP)

Profiling of the immunotoxic chemicals by a combination of Multi-ImmunoTox Assay (MITA) and IL-8 Luc assay: now in the international validation supported by JaCVAM
Setsuya Aiba (Tokio/JP)
13.30–14.00
Room
Lunch, poster viewing and exhibition
Foyer
14.00–15.00
Room
Chairs
Student session 1 – Selected abstracts
Main auditorium
Leonora Buzanska (Warsaw/PL), Costanza Rovida (Konstanz/DE)
15.00–16.30
Room
Chairs
Session 3 – Models, biomarkers and assays for systemic toxicity testing
Main auditorium
Jan Hengstler (Dortmund/DE), Sabine Ploettner (Bochum/DE)
 Hepatotoxicity: possibilities and limitations of in vitro prediction and comparison to the in vivo situation
Jan Hengstler (Dortmund/DE)
16.10–16.303D human liver spheroids for predicting drug-induced liver injury
Feng Li (Boston, MA/US)
17.00–18.30
Room
Chairs
Session 4 – Disease models and translational toxicology
Main auditorium
Heike Walles (Würzburg/DE), Daniel Dietrich (Konstanz/DE)
 Complex human tissue models and their application
Heike Walles (Würzburg/DE)
18.30–19.30
Room
Chair
Student session 2 – Career session
Main auditorium
Mathieu Vinken (Brussels/BE)
Panel MembersChantra Eskes (Magliaso/CH)
João Barroso (Ispra, IT)
Hennicke Kamp (Ludwigshafen/DE)
Helena Kandárová (Bratislava/SK)
Laura Suter-Dick (Basel/CH)
Roland Buesen (Ludwigshafen/DE)
19.30–20.30
Room
SSCT general assembly
Main auditorium
08.30–10.30
Room
Chairs
Session 5 – New developments in local toxicity and skin sensitisation testing
Main auditorium
Natalie Alépée (Aulnay-Sous-Bois/FR), Sebastian Hofmann (N./DE)
 An end-user vision on the benefits of alternative methods in skin sensitisation testing
Natalie Alépée (Aulnay-Sous-Bois/FR)
11.00–12.30
Room
Chairs
Session 6 – In silico modelling and read-cross approaches
Main auditorium
Eann A. Patterson (Liverpool/GB), Laura Suter-Dick (Basel/CH)
 Establishing credibility for predictive toxicology approaches
Eann A. Patterson (Liverpool/GB)
12.30–13.30
Room
Chairs
Lunch session – InnoSysTox – Innovative Systems Toxicology for alternatives to animal testing
Main auditorium
Sonja Matthiesen (Jülich/DE), Rob Diemel (AE The Hague/NL)
The onconoid hub – Targeting liver tumors with organoid systems biology
Bruno Christ (Leipzig/DE)
Systems biology of liver toxicity predictions
Rob van de Water (Leiden/NL)
Integration of mechanism-based in vitro methods and quantitive in vitro-in vivo extrapolation (QIVIVE) modelling for prediction of nephrotoxicity
Angela Mally (Würzburg/DE)
13.30–15.00
Room
Chairs
Eu-ToxRisk session
Main auditorium
Bob van de Water (Leiden/NL)
The application of an imaging-based platform of adaptive stress response reporters for the assessment of liver toxicity liabilities
Bob van de Water (Leiden/NL)
Functional endpoint to screen for developmental neurotoxicity
Marcel Leist (Konstanz/DE)
A new in vitro 3D liver steatosis model for the assessment of compound exposure risks associated with fatty liver
Wolfgang Moritz (Zurich/CH)
NAMs for Hazard Assessment – systems approaches and industry perspective
Hennicke Kamp (Ludwigshafen am Rhein/DE)
15.00–16.30
Room
Chairs
In3 session
Main auditorium
Paul Jennings (Amsterdam/NL)
17.00–18.00
Room
ESTIV general assembly
Main auditorium
19.30–22.30
Location

Social evening
Restaurant Nolle*

*additional fee/ registration is necessary

08.30–10.30
Room
Chair
Session 7 – Updates and developments in regulatory toxicology
Main auditorium
Simone Hoffmann-Dörr (Düsseldorf/DE)

Challenges and opportunities for regulatory acceptance of 3R methods for safety testing
Sonja Beken (Brussels/BE)
11.00–12.30
Room
Chairs
Session 8 – New developments in inhalation toxicity testing
Main auditorium
Barbara Rothen-Rutishauser (Fribourg/CH), Robert Landsiedel (Ludwigshafen/DE)
 Predictive 3D lung models to assess the hazard of inhaled nanomaterials
Barbara Rothen-Rutishauser (Fribourg/CH)
12.30–13.00
Room
Closing
Main auditorium

Postconference workshop


The postconference workshop will combine theoretical introductions as well as practical trainings of 3 topics relevant to the fields of in vitro and in silico toxicology.

In vitro skin irritation testing
(Helena Kandárová and Silvia Letasiova, MatTek In Vitro Life Science Laboratories, Bratislava, Slovak Republic)

Participants will be given an overview of existing models and protocols for assessment of skin irritation of chemicals, cosmetics and medical devices, including their validation status and regulatory acceptance. The practical training will be focused on the handling of the reconstructed epidermis model EpiDerm™ as well as on different application techniques required in the different protocols. Each participant will get the opportunity to test a solid, a liquid and a semi-solid substance on the reconstructed epidermis.

Organ-on-a-chip modeling 
(Isabel Rütschle, Daniel Faust and Reyk Horland, TissUse GmbH, Berlin, Germany)

Participants will be given an overview of the organ-on-a-chip field, including examples of established organ models and organ combinations for use in substance testing. Aspects of qualification and validation will also be discussed. The practical training will be focused on the use and handling of multiorgan-chip devices. By setting up a skin-liver co-culture model, participants will acquire insight into the preparation of chips, cells and organ models, the experimental set-up and loading of the chip, substance exposure, sampling and in-process analytics, and endpoint controls.

In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
(Nynke Kramer, Utrecht University, The Netherlands)

Participants will be given an introduction to the mathematical basis and applications of physiologically based kinetic (PBK) modeling and quantitative in vitro-in vivo extrapolation (QIVIVE). During the practical training, participants will be able to apply this knowledge by constructing their own PBK model of toluene in rats and humans, and applying it to extrapolate in vitro neurotoxic effect concentrations to dose-equivalent levels of human exposure.

Thursday, 18 October 2018: Theoretical introductions (Main auditorium)
14.30–16.30Skin irritation testing and organ-on-a-chip models
16.30–16.45Break
16.45–17.30In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
Friday, 19 October 2018: Demonstrations and hands-on training (Nizza room)
8.30–10.30Skin irritation testing and organ-on-a-chip models 
10.30–10.45Break
10.45–12.30Skin irritation testing and organ-on-a-chip models
12.30–13.00Lunch
13.00–15.00In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
15.00Wrap-up, handing over of training certificates with photographing, adjourn

Participants can register for attending either only the theoretical introductions (Thursday 18 October 2018) or both the theoretical introductions and the practical training (Thursday 18 October and Friday 19 October 2018), at a cost of 40€ or 85€, respectively. The registration fee includes a booklet, coffee breaks and lunch (only served on Friday 19 October 2018). While there is no limitation in the number of participants attending the theoretical introductions, only 25 persons can register for both the theoretical introductions and the practical training. Registration only for the practical training is not possible. The language of the postconference workshop will be English with no simultaneous translation to other languages. Persons attending the practical training need to bring their own portable computer for the in silico training.