20th International Congress
on In Vitro Toxicology (ESTIV2018)

New approach methodologies for in vitro toxicology applications
15-18 October 2018 • Berlin / Germany

20th International Congress
on In Vitro Toxicology (ESTIV2018)

New approach methodologies for in vitro toxicology applications
15-18 October 2018 • Berlin / Germany

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Scientific programme

Preconference workshop, 15 October 2018

09.00–11.00
Room
Chairs
Preconference workshop 1 (ESTIV/JRC): AOPs: an essential tool for in vitro toxicology
Main auditorium
Maurice Whelan (Ispra/IT), Mathieu Vinken (Brussels/BE)
Panel members Mirjam Luijten (Utrecht/NL)
Brigitte Landesmann (Ispra/IT)
Dan Villeneuve (Duluth, MN/US)
Annamaria Carusi (Sheffield/UK)
11.00–11.30 Coffee break
11.30–13.30
Room
Chairs
Preconference workshop 2 (ESTIV/CAAT-EU): Good in vitro method practices/Good cell culture practice (GIVIMP/GCCP)
Main auditorium
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.30–11.40 Introduction
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.40–12.10 Overview of the OECD guidance document on GIVIMP
Sandra Coecke (Ispra/IT)
12.10–12.30 Fetal bovine serum-free media and serum alternatives
Jan van der Valk (Utrecht/NL)
12.30–12.50 GCCP – past, present and future
David Pamies (Baltimore/US)
12.50–13.10 GCCP and human pluripotent stem cell lines
Glyn Stacey (Hertfordshire/GB)
13.10–13.30 Discussion with public and panel
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)

Congress programme, 15-18 October 2018

09.00–11.00
Room
Chairs
Preconference workshop 1 (ESTIV/JRC): AOPs: an essential tool for in vitro toxicology
Main auditorium
Maurice Whelan (Ispra/IT), Mathieu Vinken (Brussels/BE)
  Mirjam Luijten (Utrecht/NL)
  Brigitte Landesmann (Ispra/IT)
  Dan Villeneuve (Duluth, MN/US)
  Annamaria Carusi (Sheffield/GB)
11.00–11.30 Coffee break
11.30–13.30
Room
Chairs
Preconference workshop 2 (ESTIV/CAAT-EU): Good in vitro method practices/Good cell culture practice (GIVIMP/GCCP)
Main auditorium
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.30 Introduction
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
11.40 Overview of the OECD guidance document on GIVIMP
Sandra Coecke (Ispra/IT)
12.10 Fetal bovine serum-free media and serum alternatives
Jan van der Valk (Utrecht/NL)
12.30 GCCP – past, present and future
David Pamies (Baltimore/US)
12.50 GCCP and human pluripotent stem cell lines
Glyn Stacey (Hertfordshire/GB)
13.10 Discussion with public and panel
Thomas Hartung (Baltimore/US), Chantra Eskes (Magliaso/CH)
13.30–20.30
Room
Posters
Foyer
13.30–14.00
Room
Lunch, poster viewing and exhibition
Exhibition
14.00–15.15
Room
Chair
Opening and keynote lecture
Main auditorium

  Welcome words
Mathieu Vinken (Brussels/BE), Robert Landsiedel (Ludwigshafen/DE), Thomas Hartung (Baltimore/US)
  Introduction
Mathieu Vinken (Brussels/BE)
Bioengineering the next generation of 3D cell models
Paula Alves (Oeiras/PT)
15.15–16.00
Room
Chair
Björn Ekwall award ceremony and lecture
Main auditorium
Stina Oredsson (Lund/SE)
15.15 Introduction
Stina Oredsson (Lund/SE)
15.20
AL
Mechanism-driven estimation of chemically induced eye nociception and irritation
Anna Forsby (Stockholm/SE)
16.30–19.00
Room
Chairs
Session 1 – Bio-engineering and stem cell models
Main auditorium
Glyn Stacey (Hertfordshire/GB), Joao Barroso (Ispra/IT)
16.30
IL 01
Building qualified tissue using advanced in vitro methods
Glyn Stacey (Hertfordshire/GB)
16.55
ST 01
Application of 3D liver microtissues for assessment of drug-induced liver injury
Tomas Hejhal (Schlieren/CH)
17.15
OP 01
Applying multi-organ-chip technologies for predictive substance testing
Ilka Maschmeyer, Jochen Kühnl, Roland Lauster, Uwe Marx (Berlin/DE)
17.36
OP 02
Credibility/validity of complex in vitro models – A crowdsource of opinions to improve their acceptance
Sofia Batista Leite, Madalena Cipriano, Donatella Carpi, Maurice Whelan, João Barroso (Ispra/IT)
17.57
OP 03
Comparison of drug sensitivities in iPSC-derived renal, liver, brain and endothelial cells using knock-out drug transporter iPSC lines
Anja Wilmes (Amsterdam/NL), Florian Meier (Biberach an der Riss/DE), Vidya Chandrasekaran, Ruthmila Paskel (Amsterdam/NL), Anwar Anzad Palakkan (Edinburgh/GB), Jessica Moser (Darmstadt/DE), Shailesh Gupta (Mölndal/SE), Philip Hewitt (Darmstadt/DE), Marie Gabrielle Zurich (Lausanne/CH), Jim Ross (Edinburgh/GB), Paul Jennings (Amsterdam/NL)
18.18
OP 04
Development and automation of 3D innovative hiPSC-based models including the microenvironment for phenotypic screening – Application on metabolic diseases
Méryl Roudaut (Nantes, Lille/FR), Amandine Caillaud, Aurélie Thédrez, Wieneke Dijk, Aurore Girardeau, Matthieu Pichelin, Lucie Arnaud, Mikaël Croyal, Cédric Le May (Nantes/FR), Élodie Vandenhaute, Zied Souguir, Nathalie Maubon (Lille/FR), Bertrand Cariou, Karim Si-Tayeb (Nantes/FR)
18.39
OP 05
iPSC-derived human 3D brain model to study developmental neurotoxicity adverse outcome pathways (DNT-AOP)
David Pamies, Carolina Nunes, Denise Tavel, Marie-Gabrielle Zurich (Lausanne/CH)
19.00–20.30
Room
Welcome reception
Exhibition
08.30–19.40
Room
Posters
Foyer
08.30–10.30
Room
Chairs
Session 2 – Toxicokinetics and in vitro-in vivo extrapolation
Main auditorium
Nynke Kramer (Utrecht/NL), Paul Jennings (Amsterdam/NL)
08.30
IL 02
In vitro biokinetic considerations in quantitative in vitro-in vivo extrapolation studies
Nynke Kramer (Utrecht/NL)
08.55
ST 02
Simulating foetal drug exposure – Sildenafil as an example
Pieter Annaert (Niel/BE)
09.15
OP 06
Prediction of adverse male reproductive health effects by integrating in vitro data and physiologically-based kinetic modelling
Anne Marie Vinggaard, Camilla Taxvig (Kgs.Lyngby/DK), Martin Scholze, Andreas Kortenkamp (Oxbridge/DE), Julie Boberg, Terje Svingen (Kgs.Lyngby/DK), Sibylle Ermler (Oxbridge/DE), Susan Strange Hermann, Anne Kruse Lykkeberg, Mikael Pedersen (Kgs.Lyngby/DK)
09.30
OP 07
PBK-based quantitative in vitro-in vivo extrapolation (QIVIVE) as critical tool in the development of alternatives to animal testing
Ans Punt, Ron Hoogenboom, Ad Peijnenburg (Wageningen/NL)
09.45
OP 08
Quantification of intracellular accumulation of lysosomotropic macrocyclic compounds by high throughput imaging of lysosomal changes
Arrabi Easwaranathan, Beril Inci, Sam Ulrich, Lars Brunken, Violetta Nikiforova, Ulf Norinder (Södertälje/SE), Stephen Swanson (Stevenage/GB), Vesna Munic Kos (Södertälje/SE)
10.00
OP 09
Bioavailability of organic micropollutants in cell-based bioassays
Fabian Fischer, Luise Henneberger, Rita Schlichting (Leipzig/DE), Beate Escher (Leipzig, Tübingen/DE)
10.15
OP 10
Characterisation of chemical metabolism in a multi organ chip device integrating skin and liver models
Jochen Kühnl (Hamburg/DE), Ilka Maschmeyer (Berlin/DE), Katrin Brandmair (Hamburg/DE), Thi Phuong Tao (Berlin/DE), Silke Gerlach (Hamburg/DE), Ursula Müller-Vieira (Saarbrücken/DE), Hélèn Duplan (Toulouse/FR), Joan Eilstein (Aulnay-Sous-Bois/FR), Corie Ellison (Cincinnati/US), Sébastien Grégoire (Aulnay-Sous-Bois/FR), Carine Jacques-Jamin (Toulouse/FR), Martina Klaric (Brussels/BE), Daniela Lange (Hamburg/DE), Cathy Lester (Cincinnati/US), Sabrina Salhi (Nyon/CH), Ian Sorrell (Sharnbrook/GB), Uwe Marx (Berlin/DE), Andreas Schepky (Hamburg/DE), Nicola Hewitt (Brussels/BE)
11.00–12.30
Room
Chairs
Session 3 – Models, biomarkers and assays for systemic toxicity testing
Main auditorium
Aldert Piersma (Bilthoven/NL), Marcel Leist (Konstanz/DE)
11.00
IL 03
Integrated approaches to testing and assessment in developemntal toxicology
Aldert Piersma (Bilthoven/NL)
11.25
ST 03
Rapid and accurate high-throughput in vitro prediction of nephrotoxicity in human with proxtox-hts
Daniele Zink (Singapore/SG)
11.45
OP 11
Smart screening of chemicals – Evaluation of retinoic acid receptor alpha (RARα) interaction of chemicals by combining two techniques
Barbara Birk (Ludwigshafen/DE), Philipp Demuth (Ludwigshafen, Kaiserslautern/DE), Nadine Roth, Katharina Maria Sessler, Hans-Albrecht Hüner, Andreas Verlohner, Burkhard Flick, Bennard van Ravenzwaay, Robert Landsiedel (Ludwigshafen/DE)
12.00
OP 12
High-throughput in vitro quantification of intracellular liver metabolites to predict liver safety and mechanisms of drug toxicity
Delyan Ivanov, Priyanka Narasimhan, Matthew Burnham (Macclesfield/GB), Ana Teixeira (Cambridge/GB), Ian Sinclair (Macclesfield/GB), Martin Bachman (Alderley Park/GB)
12.15
OP 13
Development of MS-based immunoassays for the analysis of toxicologically relevant biomarkers in pesticide-treated HepaRG cells
Felix F. Schmidt, Andreas E. Steinhilber, Helen S. Hammer (Reutlingen/DE), Almut Mentz, Jörn Kalinowski (Bielefeld/DE), Dajana Lichtenstein, Albert Braeuning, Philip Marx-Stoelting, Alfonso Lampen (Berlin/DE), Thomas O. Joos, Oliver Poetz (Reutlingen/DE)
12.30–13.30
Room
Lunch session – Updates from JSAAE
Main auditorium
12:30
LT 01
Japanese strategy on alternative to animal test methods for systemic toxicology
Hajime Kojima (Tokio/JP)
13:00
LT 02
Profiling of the immunotoxic chemicals by a combination of Multi-ImmunoTox Assay (MITA) and IL-8 Luc assay – Now in the international validation supported by JaCVAM
Setsuya Aiba (Sendai/JP)
13.30–14.00
Room
Lunch, poster viewing and exhibition
Exhibition
14.00–15.00
Room
Chairs
Student session 1 – Selected abstracts
Main auditorium
Leonora Buzanska (Warsaw/PL), Costanza Rovida (Konstanz/DE)
14:00
SP 01
Nicotine and cotinine interfere with the cells′ antioxidative defense in smokers, by inhibiting catalase and glutathione reductase activity during osteogenic differentiation of SCP-1 cells
Romina Aspera-Werz, Sabrina Ehnert, Daniel Heid, Sheng Zhu, Tao Chen, Bianca Braun, Monja Müller, Andreas Nüssler (Tübingen/DE)
14:15
SP 02
Ethosomes containing octadecenedioic acid (ODA) for whitening cosmetic products – In vitro assessment of efficacy, skin sensitisation and photosensitisation
Larissa Moreira, Percília Lucena, Danillo Veloso, Eliana Martins, Marize Valadares (Goiânia/BR)
14:30
SP 03
Establishment of bile duct-like structure by mimicking the intrahepatic bile duct morphogenesis for an in vitro chemical and drug testing
Astia Safitri, Marie Shinohara, Minoru Tanaka, Yasuyuki Sakai (Tokyo/JP)
14:45
SP 04
Development of a high-throughput screening for oxidative DNA damage in human cells
Matthias Mack, Alexander Bürkle (Konstanz/DE)
15.00–16.30
Room
Chairs
Session 3 – Models, biomarkers and assays for systemic toxicity testing
Main auditorium
Jan Hengstler (Dortmund/DE), Sabine Plöttner (Bochum/DE)
15.00
IL 04
Hepatotoxicity – Possibilities and limitations of in vitro prediction and comparison to the in vivo situation
Jan Hengstler (Dortmund/DE)
15.25
ST 04
3D human liver spheroids for predicting drug-induced liver injury
Feng Li (Boston/US)
15.45
OP 14
Developing a novel 3-dimensional small intestinal-like model for toxicity screening
Paulus Jochems, Anne Metje van Genderen, Sophie Eis, Silvia Fattorini, Johan Garssen, Prescilla Jeurink, Jeroen van Bergenhenegouwen, Roos Masereeuw (Utrecht/NL)
16.00
OP 15
Optimisation of assays for assessing mitochondrial injury in human renal proximal tubular epithelial cells
Giada Carta, Paul Jennings (Amsterdam/NL)
16.15
OP 16
Cellular recovery and resilience – A new angle to look at toxicity testing
Lena Smirnova, Georgina Harris (Baltimore/US), Melanie Eschment (Baltimore/US, Konstanz/DE), Alexandra Maertens, Andre Kleensang, Helena Hogberg (Baltimore/US), Thomas Hartung (Baltimore/US, Konstanz/DE)
17.00–18.40
Room
Chairs
Session 4 – Disease models and translational toxicology
Main auditorium
Heike Walles (Würzburg/DE), Daniel Dietrich (Konstanz/DE)
17.00
IL 05
Complex human tissue models and their application
Heike Walles (Würzburg/DE)
17.25
OP 17
The role of autism risk gene, CHD8, in chlorpyrifos-induced neurotoxicity in iPSC-derived BrainSphere model
Lena Smirnova (Baltimore/US), Xiali Zhong (Baltimore/US, Changsha/CN), David Pamies, Helena Hogberg (Baltimore/US), Herbert Lachman (NY/US), Thomas Hartung (Baltimore/US, Konstanz/DE)
17.40
OP 18
Cancer xenografts on the chick chorioallantoic membrane for the evaluation of efficacy and toxicity of anticancer therapeutics
Tatiana Syrovets, Ann-Kathrin Gaiser, Michael Schmiech, Susanne Hafner, Volker Rasche (Ulm/DE), Tanja Weil (Mainz/DE), Thomas Simmet (Ulm/DE)
17.55
OP 19
Application of a human skin stem cell-derived in vitro liver model of non-alcoholic steatohepatitis
Joost Boeckmans, Valerie Vandenbempt, Karolien Buyl, Alessandra Natale, Vera Rogiers, Joery De Kock, Tamara Vanhaecke, Robim Marcelino Rodrigues (Brussels/BE)
18.10
OP 20
3D engineered human intestine model to replicate in vitro normal and diseased conditions of the human gut 
Giorgia Imparato, Vincenza De Gregorio, Francesco Urciuolo (Naples/IT)
18.25
OP 21
Streptococcus pneumoniae inhibits pseudomonas aeruginosa growth on nasal human epithelium in vitro
Samuel Constant, Song Huang, Carole Bertinetti, Ophélie Verbeke, Mireille Caul-Futy, Paul Alouani, Ludovic Wiszniewski (Plan les Ouates/CH)
18.40–19.40
Room
Chair

Panel members
Student session 2 – Career session
Main auditorium
Iwona Wilk-Zasadna (Basel/CH)

Chantra Eskes (Magliaso/CH)
Joao Barroso (Ispra/IT)
Hennicke Kamp (Ludwigshafen/DE)
Helena Kandarova (Bratislava/SK)
Anne Marie Vinggaard (Kgs. Lyngby/DK)
Roland Buesen (Ludwigshafen/DE)
19.40–20.40
Room
SSCT General assembly
Main auditorium
08.30–18.00
Room
Posters
Foyer
08.30–10.30
Room
Chairs
Session 5 – New developments in local toxicity and skin sensitisation testing
Main auditorium
Natalie Alépée (Aulnay-Sous-Bois/FR), Sebastian Hoffmann (Paderborn/DE)
08.30
IL 06
An end-user vision on the benefits of alternative methods in skin sensitisation testing
Natalie Alépée (Aulnay-Sous-Bois/FR)
08.55
OP 22
Dendritic cell activation induced by contact allergens of different potency – an in vitro evaluation
Valentina Galbiati, Sara Sirtori, Giorgia Iacomini, Lucrezia Santonocito, Emanuela Corsini (Milan/IT)
09.14
OP 23
Skin-on-a-chip with an automated air-liquid interface
Joachim Wiest (Kronburg, Munich/DE), Sebastian Eggert (Kronburg/DE, Kelvin Grove/AU), Frank A. Alexander (Kronburg/DE)
09.33
OP 24
The THP-1 cell toolbox – A new method that integrates the AOP 4 key events of skin sensitisation
Elodie Clouet (Toulouse/FR), Rami Bechara, Chloe Raffalli, Marie-Hélène Damiens, Marc Pallardy (Chatenay-Malabry/FR), Pierre-Jacques Ferret (Toulouse/FR), Saadia Kerdine-Römer (Chatenay-Malabry/FR)
09.52
OP 25
Human skin model to study the impact of ultraviolet radiation on skin permeation and metabolism of complex mixtures of polycyclic aromatic hydrocarbons
Etienne Bourgart, Marie Marques (La Tronche/FR), Anne Von Komschenbahr, David Béal (Grenoble/FR), Renaud Persoon (La Tronche/FR), Marie-Thérèse Leccia, Thierry Douki (Grenoble/FR), Anne Maitre (La Tronche/FR)
10.11
OP 26
In chemico evaluation of drug induced skin sensitisation and photo sensitivity using direct peptide reactivity assay (DPRA)
Nitin Patel, Priyanka Mishra, Irfan Tamboli, Rahul Date (Valvada/IN)
11.00–12.30
Room
Chairs
Session 6 – In silico modelling and read-across approaches
Main auditorium
Eann A. Patterson (Liverpool/GB), Helena Kandarova (Bratislava/SI)
11.00
IL 07
Establishing credibiliting for predictive toxicology approaches
Eann A. Patterson (Liverpool/GB)
11.25
OP 27
Prediction of toxico-genomics regulation by microRNA in zebrafish (Danio rerio) after exposure to nanoparticles
Moyan Hu, Boris Jovanovic, Dušan Palić (München/DE)
11.41
OP 28
Disruption of the estrogenic signaling by coactivator binding inhibitors – An in silico predictive approach
Elizabeth Goya-Jorge (Burjassot, Valencia, Valencia/ES), Rosa Maria Giner, Jesus Vicente de Julian-Ortiz (Burjassot, Valencia/ES), Rafael Gozalbes (Valencia/ES)
11.57
OP 29
Describing and assessing the uncertainty of in silico models for toxicity prediction
Mark Cronin (Liverpool/GB), Andrea-Niciole Richarz (Ispra/IT), Terry Schultz (Knoxville TN/US)
12.13
OP 30
Integrated testing strategy for carcinogenicity evaluation of chemicals using genotoxicity tests and chemical properties
Yurika Fujita (Tochigi/JP, Darmstadt/DE), Hiroshi Honda, Masayuki Yamane (Tochigi/JP), Takeshi Morita (Kanagawa/JP), Tomonari Matsuda (Shiga/JP), Osamu Morita (Tochigi/JP)
12.30–13.30
Room
Chairs
Lunch session – InnoSysTox – Innovative Systems Toxicology for alternatives to animal testing
Main auditorium
Sonja Matthiesen (Jülich/DE), Rob Diemel (The Hague/NL)
12.30
LT 03
The onconoid hub – Targeting liver tumors with organoid systems biology
Bruno Christ (Leipzig/DE)
12.50
LT 04
Systems biology of liver toxicity predictions
Bob van de Water (Leiden/NL)
13.10
LT 05
Integration of mechanism-based in vitro methods and quantitative in vitro-in vivo extrapolation (QIVIVE) modelling for prediction of nephrotoxicity
Angela Mally (Würzburg/DE)
12.30–13.30
Room
Lunch, poster viewing and exhibition
Exhibition
13.30–15.00
Room
Chair
Eu-ToxRisk session
Main auditorium
Bob van de Water (Leiden/NL)
13.30
PT 01
The application of an imaging-based platform of adaptive stress response reporters for the assessment of liver toxicity liabilities
Bob van de Water (Leiden/NL)
13.52
PT 02
Functional endpoint to screen for developmental neurotoxicity
Marcel Leist (Konstanz/DE)
14.14
PT 03
A new in vitro 3D liver steatosis model for the assessment of compound exposure risks associated with fatty liver
Patrick Guye (Schlieren/CH)
14.36
PT 04
NAMs for Hazard Assessment – systems approaches and industry perspective
Hennicke Kamp (Ludwigshafen/DE)
15.00–16.30
Room
Chair
In3 session
Main auditorium
Paul Jennings (Amsterdam/NL)
15.00
PT 05
Utilisation of human iPSC derived renal proximal tubular epithelial-like cells for understanding the role of the megalin transporter in nephrotoxicity
Vidya Chandrasekaran (Amsterdam/NL)
15.05
PT 06
The development and optimisation of a protocol to generate podocytes from induced pluripotent stem cells
Cormac Murphy (Amsterdam/NL)
15.10
PT 07
iPSCs differentiated to endothelial cells as model system for in vitro toxicology
Zahra Mazidi (Vienna/AT)
15.15
PT 08
Protocol comparison for deriving BBB-models from iPSCs for use in toxicological assessment of chemicals in the light of the in3 project
Sara Wellens (Lens/FR)
15.20
PT 09
iPSC-derived human 3D model (brainspheres) for neurotoxicity testing
Carolina Nunes (Lausanne/CH)
15.25
PT 10
Development of a cellular toxicology model using genetically engineered human induced pluripotent stem cells
Aurore Bourguignon (Gödöllő/HU)
15.30
PT 11
Optimisation of an iPSC-derived hepatocyte model for toxicity testing and AOP development
Sreya Ghosh (Leuven/BE)
15.35
PT 12
Development of an iPSC-derived human in vitro small airway model
Leonie Fransen (Chilton/GB)
15.40
PT 13
Optimisation of induced pluripotent stem cell (iPSC) derived lung airway models for toxicity testing and risk assessment evaluation
Ivo Djidrovski (Newcastle/GB)
15.45
PT 14
Introducing ab initio QSAR, a versatile tool for regression and classification
Kristijan Vukovic (Milan/IT)
15.50
PT 15
Development of in silico classification models to predict the toxicity of azoles as aromatase or not aromatase inhibitors
Ana Yisel Caballero (Milan/IT)
15.55
PT 16
Development and use of adverse outcome pathway (AOP) networks within the in3 project
Nicoleta Spînu (Liverpool/GB)
16.00
PT 17
P-glycoprotein modeling – A combined in silico approach towards identification of safer chemicals
Liadys Mora Lagares (Ljubljana/SI)
16.05
PT 18
Evaluation of models to estimate the distribution kinetics of test chemicals in vitro
Susana Proença (Utrecht/NL)
16.10
PT 19
Towards bioinformatics workflow for prediction of chronic kidney disease based on IPS cell data
Pranika Singh (Basel/CH)
16.15
PT 20
The in3 project – An integrated interdisciplinary approach to animal-free nanomaterial and chemical safety assessment
Paul Jennings (Amsterdam/NL)
17.00–18.00
Room
ESTIV general assembly
Main auditorium
19.30–22.30
Room
Social evening
Restaurant Nolle
08.30–10.30
Room
Chairs
Session 7 – Updates and developments in regulatory toxicology
Main auditorium
Sonja Beken (Brussels/BE), Simone Hoffmann-Dörr (Düsseldorf/DE)
08.30
IL 08
Challenges and opportunities for regulatory acceptance of 3R methods for safety testing
Sonja Beken (Brussels/BE)
08.55
OP 31
Newly validated 3D skin genotoxicity assays improve safety assessments of cosmetic ingredients
Kerstin Reisinger (Düsseldorf/DE), Marilyn J Aardema (Fairfield/US), Roger Curren (Gaithersburg/US), Mona Delagrange (Sharnbrook/GB), Bertrand Desprez (Brussels/BE), Rolf Fautz (Darmstadt/DE), Nicola J Hewitt (Brussels/BE), Sebastian Hoffmann (Paderborn/DE), Julia Kenny (Ware/GB), Jochen Kühnl (Hamburg/DE), Andreas Luch (Berlin/DE), Gavin Maxwell (Sharnbrook/GB), Gladys Ouédraogo (Aulnay-sous-Bois/FR), Ralph Pirow (Berlin/DE), Astrid A Reus (Zeist/NL), Shambu Roy (Rockville/US), Monika Schäfer-Korting (Berlin/DE), Markus Schulz (Ludwigshafen/DE), Brian A Wall (Piscataway/US), Stefan Pfuhler (Mason/US)
09.18
OP 32
Reconstructed human epithelia for in vitro biocompatibility of medical devices
Christian Pellevoisin (Lyon/FR)
09.41
OP 33
Validation and regulatory acceptance of innovative 3R approaches for the safety assessment of chemicals – Results of the second joint workshop of BfR and RIVM
Janine Ezendam (Bilthoven/NL), Tanja Burgdorf (Berlin/DE), Jan van Benthem, Anne Kienhuis (Bilthoven/NL), Michael Oelgeschläger (Berlin/DE), Aldert Piersma (Bilthoven/NL), Gilbert Schönfelder (Berlin/DE)
10.04
OP 34
An EU-NETVAL validation study to identify potential thyroid disruptors
Sandra Coecke, Francesca Pistollato, Anne Milcamps, Ingrid Langezaal, Gerard Bowe, Camilla Bernasconi, Thomas Cole, Elise Grignard, Sharon Munn (Ispra/IT)
08.30–11.00
Room
Posters
Foyer
11.00–12.40
Room
Chairs
Session 8 – New developments in inhalation toxicity testing
Main auditorium
Barbara Rothen-Rutishauser (Fribourg/CH), Robert Landsiedel (Ludwigshafen/DE)
11.00
IL 09
Predictive 3D lung models to assess the hazard of inhaled nanomaterials
Barbara Rothen-Rutishauser (Fribourg/CH)
11.25
OP 35
Respiratory toxicity testing strategy – In chemico/in vitro developments to meet challenges of inhalable substances from chemical, consumer goods and pharmaceutical industry
Udo Bock, Jennifer Hennen (Trier/DE), Marius Hittinger, Henrik Groß, Claus-Michael Lehr (Saarbrücken/DE), Barbara Birk, Lan Ma-Hock, Robert Landsiedel (Ludwigshafen/DE), Taryn Kirsch, George F. Gerberick (Schwalbach/DE), Brunhilde Blömeke (Trier/DE)
11.40
OP 36
Pre-validation of an acute inhalation toxicity test using the EpiAirway in vitro human airway model
Anna Maione, George R Jackson, Mitchell Klausner (Ashland/US), Silvia Letasiova, Helena Kandarova (Bratislava/SK), Patrick Hayden (Ashland/US)
11.55
OP 37
Particle- and fiber-induced cell migration in vitro as a novel screening tool for adverse health effects of inhalable dusts from various sources
Götz Westphal, Christian Monsé, Nina Rosenkranz (Bochum/DE), Markus Mattenklott (Sankt Augustin/DE), Bryan Hellack (Duisburg/DE), Thomas Brüning, Jürgen Bünger (Bochum/DE)
12.10
OP 38
Application of in vitro approaches for the assessment of next generation tobacco and nicotine products
Marianna Gaca, Frazer Lowe, Emmanuel Minet, David Thorne, Damien Breheny (Southampton/GB)
12.25
OP 39
Investigation of reversibility of foamy phenotype in human alveolar macrophages
Ewelina Hoffman, Victoria Hutter (Hatfield/GB)
12.40–13.10
Room
Closing
Main auditorium
14.30–16.30
Room
Postconference workshop
Main auditorium
14.30 Skin irritation testing and organ-on-a-chip models
Helena Kandarova, Silvia Letasiova (Bratislava/SK), Isabel Rütschle, Daniel Faust, Reyk Horland (Berlin/DE)
16.30–16.45
Room
Coffee break
Exhibition
16.45–17.30
Room
Postconference workshop
Main auditorium
16.45 In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
Nynke Kramer (Utrecht/NL)
08.30–10.30
Room
Postconference workshop
Nizza
08.30 Skin irritation testing and organ-on-a-chip models I
Helena Kandarova, Silvia Letasiova (Bratislava/SK), Isabel Rütschle, Daniel Faust, Reyk Horland (Berlin/DE)
10.30–10.45
Room
Coffee break
Exhibition
10.45–12.30
Room
Postconference workshop
Nizza
10.45 Skin irritation testing and organ-on-a-chip models I
Helena Kandarova, Silvia Letasiova (Bratislava/SK), Isabel Rütschle, Daniel Faust, Reyk Horland (Berlin/DE)
12.30–13.00
Room
Lunch break
Exhibition
13.00–15.00
Room
Postconference workshop
Nizza
13.00 In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
Nynke Kramer (Utrecht/NL)

Posters

08.30–11.00
Room
Posters
Foyer
 

P 02
Developmental neurotoxicity studies using the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y
Kristina Attoff (Stockholm/SE), Anda Gliga (Stockholm/DE), Dimitra Kertika, Jessica Lundqvist (Stockholm/SE), Stina Oredsson (Lund/SE), Anna Forsby (Stockholm, Södertälje/SE)

P 03
Development of a cellular toxicology model using genetically engineered human induced pluripotent stem cells
Aurore Bourguignon, István Bock, Anita Fehér, Julianna Kobolák, András Dinnyés (Gödöllo/HU)

P 04
Evaluation of cardiac cell models for preclinical predictive studies of drug-induced cardiotoxicity
Carol De Santis, Jason Gill (Newcastle upon Tyne/GB)

P 05
Formation of 3D-spheroids in different cell lines by a new coating technology
Barbara Birk, Veronique Schwartz, Rupert Konradi, Selina Hemmer, Hans-Albrecht Hüner, Bennard van Ravenzwaay, Robert Landsiedel (Ludwigshafen/DE)

P 06
Human induced pluripotent stem cell based in vitro neurotoxicology model
Andras Dinnyes, Zofia Janstova, Balazs Mihalik, Maria Bodi-Jakus, Julianna Kobolak (Gödöllo/HU)

P 07
TCDD induces complex alterations of pathways controlling proliferation, survival and differentiation in human liver progenitor cell model
Jana Svobodová, Jiřina Procházková, Lenka Šmerdová (Brno/CZ), Helena Líbalová, Jan Topinka, Jiří Kléma (Prague/CZ), Alena Hyršlová Vaculová, Miroslav Machala, Jan Vondráček (Brno/CZ)

P 08
Establishment of human 3D liver cell cultures of different stiffness and their effect on the metabolism of drugs
Marc Ruoß, Marina Weimer, Silas Rebholz, Victor Häussling, Sabrina Ehnert, Andreas Nussler (Tübingen/DE)

P 09
Establishment of bile duct-like structure by mimicking the intrahepatic bile duct morphogenesis for an in vitro chemical and drug testing
Astia Safitri, Marie Shinohara, Minoru Tanaka, Yasuyuki Sakai (Tokyo/JP)

P 10
iPSC and iPSC-derived proximal tubule cells differ in their response to nephrotoxic substances
Cornelia Hirsch, Andreas Weber, Gerhard Fritz, James Adjaye, Nicole Schupp (Düsseldorf/DE)

P 11
Generation of proliferating human liver sinusoidal endothelial cells
Nikolett Nagy, Astrid Noerenberg, Sandra Rohrmoser, Torge Evenburg, Timo Johannssen (Hamburg/DE)

P 12
Characterisation of proliferating human hepatocytes as a model system for drug interaction studies and toxicity screenings
Nikolett Nagy (Hamburg/DE), M. José Gómez-Lechón, Ramiro Jover (Valencia/ES), Timo Johannssen (Hamburg/DE)

P 13
A high-throughput microfluidic 3D human liver tissue model for hepatotoxicity prediction
Kristin Bircsak (Leiden/NL), Richard DeBiasio (Pittsburgh/US), Ryan Reddinger (Leiden/NL), Mark Miedel (Pittsburgh/US), Karlijn Wilschut (Leiden/NL), Lawrence Vernetti, D. Lansing Taylor (Pittsburgh/US), Paul Vulto (Leiden/NL), Albert Gough (Pittsburgh/US), Anthony Saleh (Leiden/NL)

P 14
Motor neurons generated in vitro for botulinum neurotoxin potency testing vary in the expression of motor neuron markers and genes relevant for mechanism of toxicity
Maren Lück (Hannover/DE), Brit-Maren Schjeide, Gerhard P. Püschel (Nuthetal/DE), Bettina Seeger (Hannover/DE)

P 15
Optimisation of induced pluripotent stem cell (iPSC) derived lung airway models for toxicity testing and risk assessment evaluation
Ivo Djidrovski, Katie Anderson, Lyle Armstrong (Newcastle upon tyne/GB)

P 16
The development and optimisation of a protocol to generate podocytes from induced pluripotent stem cells
Cormac Murphy (Amsterdam/NL), Caroline Rauch, Elisabeth Feifel, Georg Kern, Gerhard Gstraunthaler (Innsbruck/AT), Anja Wilmes, Paul Jennings (Innsbruck/AT, Amsterdam/NL)

P 17
Functional cardiotoxicity assessment using 3D cardiac microtissue
Priyanka Narasimhan (Cheshire/GB)

P 18
Utilisation of human iPSC derived renal proximal tubular epithelial-like cells for understanding the role of the megalin transporter in nephrotoxicity
Vidya Chandrasekaran, Anja Wilmes, Paul Jennings (Amsterdam/NL)

P 20
Impact of doxorubicin on differentiation of stem cells into endothelial-like cells
Sarah Kim Jahn, Gerhard Fritz (Düsseldorf/DE)

P 21
Impact of scaffolds and fluidics on the hepatic differentiation of human skin precursors
Alessandra Natale, Koen Vanmol (Brussels/BE), Aysu Arslan (Ghent/BE), Sandra Van Vlierberghe (Ghent, Brussels/BE), Peter Dubruel (Ghent/BE), Jürgen Van Erps, Hugo Thienpont, Joost Boeckmans, Joery De Kock, Vera Rogiers, Robim M. Rodrigues, Tamara Vanhaecke (Brussels/BE)

P 22
Tissue-like platform for cancer cells obtained with in vitro formed vasculature
Tuula Heinonen, Outi Huttala, Emilia Koskinen, Johanna Mäenpää, Minna Tanner, Timo Ylikomi (Tampere/FI)

P 23
iPSC-derived human 3D model (BrainSperes) for neurotoxicity testing
Carolina Nunes, David Pamies, Marie-Gabrielle Zurich (Lausanne/CH)

P 24
Mass production of induced pluripotent stem cell derived proximal tubule epithelial cells
Thao Ngo, Krithika Hariharan, Andreas Kurtz (Berlin/DE)

P 25
The effect of zidovudine (AZT) on autophagy in C2C12 myocytes
Hazel Lin, Metodi Stankov, George Behrens (Hannover/DE)

P 26
COPD and asthma therapeutics for supportive treatment in organophosphate poisoning – A study in rat PCLS
Julia Herbert, Franz Worek, Horst Thiermann, Timo Wille (Munich/DE)

P 27
Development of a novel human 3D in vitro model for evaluating new anti-fibrotic drugs
Amanda Woodrooffe, Caroline Freathy, Samantha Maitland, Kathryn Bagot, Howard Loraine, Paul Murdock (Royston/GB)

P 28
In vitro evaluation of cell signalling processes associated with the potential genotoxicity of metal oxide nanoparticles in physioxic culture conditions
Michael K.T. Theodoulides (Swansea, Cardiff/GB), Martin J.D.Clift (Swansea/GB), Rachel Adams (Cardiff/GB), Stephen J.Evans (Swansea/GB), Sandor Balog (Fribourg/CH), Nicole Hondow, Andy Brown (Leeds/GB), Richard Webb (Cardiff/GB), Shareen H. Doak (Swansea/GB)

P 29
A human proximal tubule epithelial cell/fibroblast transwell co-culture system
Felicitas Piossek, Sabrina Henke, Nadja Schlichenmaier, Sascha Beneke, Daniel Dietrich (Konstanz/DE)

P 30
p, p"-DDT induce apoptosis in human endometrial stromal cells through oxidative stress
Seung Bin Park, Yeon Jean Cho, Myoungseok Han (Busan/KR)

P 31
Development of an in vitro model for the investigation of pathogenicity mechanisms of gut diseases caused by zoonotic pathogens
Pascal Hoffmann, Michael T. Empl, Johannes Junginger, Wencke Reineking, Marion Hewicker-Trautwein, Gerhard Breves (Hannover/DE)

P 32
The PODO/TERT256 cell line – A novel tool for in vitro modelling of glomerulotoxicity
Nadja Schlichenmaier, Rebecca Bohnert, Felicitas Piossek, Sascha Beneke, Daniel Dietrich (Konstanz/DE)

P 33
Cell transformation assays – Their use goes beyond the assessment of chemicals' carcinogenesis
Monica Oldani, Matilde Forcella, Pasquale Melchioretto (Milano/IT), Marco Fabbri, Laura Gribaldo (Ispra/IT), Marta Lucchetta, Isabelle da Piedade, Elena Papaleo (Copenaghen/DK), Chiara Urani, Paola Fusi (Milano/IT)

P 34
Organotypic in vitro human airway models can recapitulate aspects of pulmonary fibrosis
Anna Maione, George R Jackson, Olivia O'Connell, Jaclyn Webster, Mitchell Klausner (Ashland/US), Silvia Letasiova, Helena Kandarova (Bratislava/SK), Patrick Hayden (Ashland/US)

P 35
An innovative full-thickness human skin model with a responsive ECM to study time and space evolution of dermis during wound closure
Francesco Urciuolo (Napoli/IT), Giorgia Imparato (Napoli/IT), Costantino Casale (Napoli/IT), Paolo Netti (Napoli/IT)

P 36
Cutaneous melanoma model in vitro recreate the tumor environment during invasion process
Costantino Casale, Bernadette Lombardi, Giorgia Imparato, Francesco Urciuolo, Paolo Netti (Napoli/IT)

P 37
Preclinical implications for GDF15-mediated stemness and chemoresistance in type I human epithelial ovarian cancer cells
Ki Hyung Kim (Busan/KR), Yuseok Moon (Yangsan/KR)

P 38
A translational model to assess immunogenicity and predict drug induced immune mediated secondary disease
Masih Alam, Rawshan Choudhury, Robert-Jan Lamers (Macclesfield/GB)

P 39
Applying an in vitro co-culture model of the healthy and inflamed intestine to study nanomaterial-induced toxicity and barrier translocation
Angela Kämpfer (Düsseldorf/DE, Ispra/IT, Edinburgh/GB), Patricia Urbán, Rita La Spina, Uwe Holzwarth, Isaac Ojea Jiménez, Elena Bellido, Carine Drewes (Ispra/IT), Vicki Stone (Edinburgh/GB), Agnieszka Kinsner-Ovaskainen (Ispra/IT)

P 40
Deleterious effects of the herbicide glufosinate-ammonium and its main metabolite on neural stem cells from newborn mouse subventricular zone
Justyne Feat-Vetel, Vanessa Larrigaldie (Orleans/FR), Thierry Gefflaud (Clermont-Ferrand/FR), Stéphane Mortaud (Orleans/FR)

P 41
Identification of mechanisms involved in drug-induced cholestasis using HepaRG hepatocytes
Audrey Burban, Ahmad Sharanek, Andre Guillouzo, Christiane Guguen-Guillouzo (Rennes/FR)

P 42
Protective effect of silymarin against acute myeloid leukemia (AML) through downregulation of tunneling nanotube (TNT) and NF-κB inhibition
Aziz Eftekhari (Maragheh/IR)

P 43
Time for good practice in human stem cells neuronal differentiation
Marco Straccia, Andrea Comella Bolla, Phil Sanders, Georgina Bombau, Mireia Galofré, Josep M Canals (Barcelona, Madrid/ES)

P 44
New psychoactive substance (NPS) toxicity in zebrafish larvae
Therese Montgomery, Claire Leyden (Athlone/IE), Gethin McBean, Breandán Kennedy (Dublin/IE)

P 45
The synthetic cannabinoid R-(+)-WIN55,212-2 displays selective cytotoxic effects on Glioblastoma cell lines
Ana Gabriela Silva, Caio Fabio Lopes (Braga/PT), Rui Reis (Braga/PT, Barretos/BR), Rosy Iara Ribeiro (Braga/PT)

P 46
Characterisation of patient-derived liver organoids for precision medicine in primary liver cancer
Michael Koch, Ernst H. K. Stelzer, Francesco Pampaloni (Frankfurt am Main/DE)

P 47
Mechanistic insights into the neuroprotective and trophic effects of bacopa monniera extract against amyloid beta-peptide and hydrogen peroxide-induced toxicity
Manjeet Singh (Laval/CA)

P 48
Modelling NASH for drug discovery using 3D liver microtissues
Simon Ströbel, Jana Rupp, Sabrina Steiert, Patrick Guye, Simon Messner, Radina Kostadinova (Schlieren/CH)

P 49
Derivation of a human estimate of toxicity for the ultra-potent opioid carfentanil using PBPK modelling
Michael Feasel, Richard Lawrence, Theodore Moran, Christopher Whalley, Robert Kristovich (APG/US)

P 50
Glyphaea brevis – In vitro antioxidants and in silico biological activity of major constituents and molecular docking analyses
Janet Olugbodi (Akure/NG), Marius Tincho, Oluwafemi Oguntibeju (Cape Town/ZA), Mary Olaleye, Afolabi Akinmoladun (Akure/NG)

P 51
In silico genotoxicity and carcinogenicity prediction for food-relevant secondary plant metabolites
Josephin Glück, Thorsten Buhrke, Falko Frenzel, Albert Braeuning, Alfonso Lampen (Berlin/DE)

P 52
Bioaccumulation potential in air breathing terrestrial animals – Xenobiotic elimination half-life correlations among rat tissues
Tim Hofer, Oddvar Myhre (Oslo/NO), Doris Hirmann (Helsinki/FI), Sjur Andersen (Oslo/NO), Tiina Lantto, Johanna Peltola-Thies (Helsinki/FI)

P 53
Risk characterisation of cyclic polyamide oligomers in food in the absence of toxicological data – an in silico / read across approach
Sebastian Zellmer, Ingo Ebner, Oliver Kappenstein, Thomas Tietz, Andreas Luch (Berlin/DE)

P 54
Derivation of a TDI for the oral uptake of nickel by means of benchmark dose modelling of data from a two generation study on reproduction toxicity
Thomas Tietz, Sebastian Zellmer, Ingo Ebner, Andreas Luch (Berlin/DE)

P 55
In silico vs. in vitro comparison of nuclear receptor activation by food contaminants
Albert Braeuning (Berlin/DE), Ivano Eberini (Milano/IT), Dajana Lichtenstein, Alfonso Lampen (Berlin/DE)

P 56
Chemotype-enrichment workflow – A univariate analysis workflow for exploring chemical feature enrichments across EPA's ToxCast chemical-assay landscape
Ryan Lougee, Ann Richard, Chris Grulke (Durham/US)

P 57
Automation of acute toxicity estimate (ATE) calculations for agrochemical plant protection products (PPP)
William Masinja, Claire Elliot, Sandeep Modi, Richard Currie, Konstantinos Papachristos (Bracknell/GB)

P 58
Exploitation of ToxCast data in the safety risk assessment of food chemicals
Ans Punt (Wageningen/NL), Alan Boobis (London/GB), Mark Cronin (Liverpool/GB), Sylvain Etter (Meyrin/CH), James Firman (Liverpool/GB), Karma Fussell (Lausanne/CH), John Paul Gosling (Leeds/GB), Paul Hepburn (Colworth/GB), Agnes Karmaus (Raleigh/US), Anette Thiel (Kaiseraugst/CH), Heinz Traussnig (Vienna/AT), Martin Wilks (Basel/CH), Bettina Schelkle (Brussels/BE)

P 59
New Approach methods (NAMs) to support a read across strategy for methyl, ethyl and propyl 4-hydroxybenzoate
Susann Fayyaz (Sulzbach/DE), Jan G Hegstler, Florian Seidel (Dortmund/DE), Reinhard Kreiling (Sulzbach/DE)

P 60
Towards bioinformatics workflow for prediction of chronic kidney disease based on IPS cell data
Pranika Singh, Thomas Exner (Basel/CH)

P 61
Development and use of adverse outcome pathway (AOP) networks to support assessment of organ level effects
Nicoleta Spinu, Mark TD Cronin, Steve J. Enoch, Judith C. Madden (Liverpool/GB)

P 62
In silico prediction of mixture toxicity – Current use and future perspectives for (Q)SARs
Mark Cronin, Steven Enoch, James Firman, Judith Madden, Samuel Belfield (Liverpool/GB)

P 63
Improving chemical representation with deep learning enables skin sensitisation QSAR models
Davy Guan, Slade Matthews (Sydney/AU)

P 64
KnowTox – Risk assessment by automated read-across and machine learning including confidence estimation
Andrea Morger (Berlin/DE), Janosch Achenbach, Miriam Mathea, Antje Wolf, Roland Buesen, Klaus-Jürgen Schleifer, Robert Landsiedel (Ludwigshafen/DE), Andrea Volkamer (Berlin/DE)

P 65
In silico modelisation of compounds interaction with Bile salt Export Pump (BSEP) þ An alternative approach to predict hepatotoxicity
Solenne Martin, Damien Thomas, Ashwani Sharma (Saint Grégoire/FR)

P 66
Mutagenic evaluation of cylindrospermopsin-microcystin-LR mixtures in mouse thymic lymphoma cells and salmonella typhimurium strains
Leticia Diez-Quijada, Ana Isabel Prieto, Maria Puerto, Angeles Jos, Ana Maria Cameán (Sevilla/ES)

P 67
Application of the adverse outcome pathways (AOP) concept to systemic toxicity assessment in vitro – Kidney injury due to lysosomal overload and inhibition of mtDNA polymerase-γ as case studies
Sebastian Jarzina, Pia Reiser, Julian Scherer (Würzburg/DE), Bernhard Ellinger (Hamburg/DE), Stefano Di Fiore (Aachen/DE), Angela Mally (Würzburg/DE)

P 68
In vitro toxicity evaluation of stilbene extracts for their potential use as antioxidant in the wine industry
Concepción Medrano-Padial, María Puerto, Ana Isabel Prieto Ortega (Sevilla/ES), Emma Cantos-Villar (Jerez de la Frontera/ES), Tristán Richard (Villenave d'Ornon/FR), Ana Maria Cameán, Silvia Pichardo (Sevilla/ES)

P 69
Can metabolome analysis in vitro predict nephrotoxicity and its mode of action?
Barbara Birk, Saskia Sperber, Hans-Albrecht Hüner, Andreas Verlohner (Ludwigshafen/DE), Svenja Wallisch (Kaiserslautern, Ludwigshafen/DE), Tilmann Walk, Volker Haake, Michael Spitzer (Berlin/DE), Hennicke Kamp, Bennard van Ravenzwaay (Ludwigshafen/DE)

P 70
The impact of targeted AhR disruption on endocrine-disrupting activities of environmental chemicals in estrogen-sensitive cells
Jan Vondracek, Martina Hyzdalova, Ondrej Zapletal, Jakub Pivnicka, Martina Karasova, Katerina Pencikova (Brno/CZ), Jason Matthews (Oslo/NO), Miroslav Machala (Brno/CZ)

P 71
Clinical and bio-chemical changes in workers exposed to organic solvents
Sonja Ketin, Rade Biocanin (Novi Sad/RS)

P 72
In vitro immunohistochemistry assay for the identification of localised or systemic toxicity due to anti-drug Aatibody immune complex disease in preclinical studies testing biotherapeutics
James Raymond (Frederick/US)

P 73
Long term culture of nasal-tracheal-bronchial and bronchiolar human airway epithelia at interconnected and dynamic liquid flow conditions – The SupAir project
Samuel Constant, Mireille Caul-Futy, Song Huang, Ludovic Wiszniewski (Plan les Ouates/CH), Gregory Fischler, Adrien Roux, Luc Stoppini (Geneva/CH), Paul Alouani (Plan les Ouates/CH)

P 74
Inflammation and the AOP framework – a challenge
Brigitte Landesmann (Ispra/IT)

P 75
Transcriptional profiling of lactic acid treated reconstructed human epidermis reveals predictive biomarker for skin stinging
Md Zobaer Hasan, Yu Kawai, Amane Inoue (Kizugawa/JP)

P 76
Lipid profiling of PLHC-1 cells grown under reduced concentrations of fetal bovine serum
Anna Marqueño, Maria Blanco, Elisabet Pérez-Albaladejo, Cinta Porte (Barcelona/ES)

P 78
Fingerprinting the effects of plastic additives in the lipidome of human placenta JEG-3 cells
Elisabet Pérez-Albaladejo, Anna Marqueño, Ilaria Bani, Cinta Porte (Barcelona/ES)

P 79
Sensing of labile glycoprotein-lipid complexes in rat serum reflecting putative toxicological states
Günter Müller (Oberschleissheim/DE), Andreas Herling (Frankfurt am Main/DE), Kerstin Stemmer, Matthias Tschöp (Oberschleissheim/DE)

P 80
3D spheroids of HepaRG cells – A good model to investigate the genotoxicity of chemicals?
Marion Mandon, Sylvie Huet, Estelle Dubreil, Valérie Fessard, Ludovic Le Hégarat (Fougères/FR)

P 81
The FCS-free database for better in vitro methods
Jan van der Valk, Saskia Kliphuis (Utrecht/NL)

P 82
In vitro and in silico study of agonistic and antagonistic activities of 29 persistent organic pollutants (POPs) towards the rat and human aryl hydrocarbon receptor (AhR)
Thi Que Doan (Liège/BE), Elizabeth Goya-Jorge (Valencia/ES), Marc Muller (Liège/BE), Lisa Connolly (Belfast/GB), Rafael Gozalbes (Valencia/ES), Marie-Louise Scippo (Liège/BE)

P 83
3D human liver microtissues vs. 2D monolayer culture as an in vitro tool for compound testing
Catherine Meßner, Laura Suter-Dick (Muttenz/CH)

P 84
Assessment of iron oxide nanoparticles toxicity on cells and proteins in vitro
Olha Lahutina, Nataliia Dmyutrukha (Kyiv/UA)

P 85
Applying discovery toxicology approaches for prediction of developmental phenotypes – a case study
Kathryn Wolton, Emma Barnes, Richard Currie, Theresa Placke (Berkshire/GB)

P 86
Acute and cumulative cytotoxicity with morphine sulfate alters epigenetic genomic DNA methylation in mouse embryonic stem cells
Anirudh J. Chintalapati, Praneetha A. Panthagani, Frank A. Barile (New York/US)

P 87
In vitro bioassay toolbox for AOP-driven testing of steatosis
Dajana Lichtenstein, Claudia Luckert, Albert Braeuning (Berlin/DE), Georges de Sousa (Toulouse/FR), Sigrid Durinck (Genth/BE), Efrosini S. Katsanou, Parthena Konstantinidou, Kyriaki Machera (Athens/GR), Emanuela S. Milani (Zurich/CH), Ad Peijnenburg (Wageningen/NL), Roger Rahmani (Toulouse/FR), Andreja Rajkovic (Genth/BE), Deborah Rijkers (Wageningen/NL), Anastasia Spyropoulou (Athens/GR), Marianna Stamou (Zurich/CH), Geert Stoopen (Wageningen/NL), Shana J. Sturla, Bernd Wollscheid (Zurich/CH), Nathalie Zucchini-Pascal (Toulouse/FR), Alfonso Lampen (Berlin/DE)

P 88
Generation of recombinant human anti-diphtheria toxin neutralising antibody to replace equine sera
Esther Wenzel (Braunschweig/DE), Paul Stickings (Potters Bar/GB), Jeffrey Brown (London/GB), Thea Sesardic (Potters Bar/GB), Androulla Efstratiou (London/GB), Michael Hust (Braunschweig/DE)

P 89
Transcriptomic and proteomic analyses of HepG2 cells treated with polybrominated diphenyl ethers congeners at dietary relevant concentrations
Marialuisa Casella, Cinzia La Rocca (Rome/IT), Monia Perugini (Teramo/IT), Marco Crescenzi, Sabrina Tait (Rome/IT)

P 90
In vitro intestinal model Caco-2/HT-29-MTX to investigate luminal and basolateral factors influencing intestinal zinc resorption
Maria Maares, Claudia Keil, Hajo Haase (Berlin/DE)

P 91
3D spheroids from human neurons and astrocytes as new in vitro model to assess CNS cytotoxicity of nanoparticles
Uliana De Simone, Marianna Roccio (Pavia/IT), Francesca Caloni (Milan/IT), Arsenio Spinillo, Maria Antonietta Avanzini, Teresa Coccini (Pavia/IT)

P 92
Assessment of the genotoxic profile of a cylindrospermopsin-microsystin-LR mixture by the micronucleus and comet assays
Leticia Diez-Quijada, Maria Puerto, Ana Isabel Prieto, Ana Maria Cameán, Angeles Jos (Sevilla/ES)

P 93
Protection and reversion capacity of H202 effects of two rich extracts in stilbenes in Caco-2 and HepG2 cells
Concepción Medrano-Padial, María Puerto, Ana Isabel Prieto Ortega (Sevilla/ES), Emma Cantos-Villar (Jerez de la Frontera/ES), Tristán Richard (Villenave d'Ornon/FR), Ana Maria Cameán, Silvia Pichardo (Sevilla/ES)

P 94
Cytotoxicity and oxidative stress induced by pure Cylindrospermopsin, Aphanizomenon ovalisporum and Cylindrospermopsis raciborskii extracts in the human neuronal SH-SY5Y cell line
Maria Gracia Hinojosa, Ana Isabel Prieto Ortega, Daniel Gutiérrez-Praena, Ángeles Jos, Ana Maria Cameán (Sevilla/ES)

P 95
Toxicological profile of pure Microcystin-LR and its mixture with cylindrospermopsin in the human neuronal SH-SY5Y cell line
Maria Gracia Hinojosa, Ana Isabel Prieto, Daniel Gutiérrez-Praena, Angeles Jos, Ana Maria Cameán (Sevilla/ES)

P 96
Development of a cell-based toxicity assay for botulinum neurotoxin in a neuronal cell line and its adaptation to human motor neurons differentiated from iPSCs
Brit-Maren Schjeide, Andrea Pathe-Neuschäfer-Rube, Frank Neuschäfer-Rube (Nuthetal/DE), Maren Lück, Bettina Seeger (Hannover/DE), Gerhard P. Püschel (Nuthetal/DE)

P 97
Reversibility of in vitro biological effects in cigarette smoke-exposed 3D lung tissues following switching to a tobacco heating product
Tomasz Jaunky, Linsey Haswell, Sarah Corke, Simone Santopietro, Andrew Baxter, Gareth Waters, Annette Dalrymple, Emmanuel Minet, David Thorne, Marianna Gaca (Southampton/GB)

P 98
Adulterated food matrix attenuates biomarkers of mycotoxin-induced barrier disruption – In vitro mechanistic evaluation of mucoactive herbal components
Yuseok Moon, Zhimin Du, Juil Kim (Yangsan/KR), Ki Hyung Kim (Busan/KR)

P 99
MicroRNA expression changes in A549 alveolar epithelial cells after 7 days in vitro incubation
Célia Ventura, Luís Vieira, Catarina Silva, António Sousa-Uva, Maria João Silva (Lisbon/PT)

P 100
Induction of epithelial-to-mesenchymal transition (EMT) on human bronchial Beas-2B cells exposed to fine particulate matter PM2.5
Elina El Hajj, Clémence Meausoone, Gauthier Tremolet, Dominique Courcot, Frédéric Ledoux, Yann Landkocz (Dunkerque/FR)

P 101
Toxicolipidomics as a novel approach in mechanistic studies on the effects of organic pollutants in vitro
Miroslav Machala, Josef Slavík, Katerina Pencíková, Pavlína Simecková, Lucie Svrzková, Jirina Procházková, Jan Vondrácek (Brno/CZ)

P 102
Advanced engineered gut-liver tissues on chip for in vitro toxicity testing
Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo, Brunella Corrado, Maria Rosaria Telesco, Paolo Antonio Netti (Napoli/IT)

P 103
An advance in vitro model based on testicular Leydig TM3 cells as a prescreening tool for testicular toxicity assessment
Iva Sovadinova, Eliska Sychrova, Petra Laboha, Amrita Basu, Affiefa Yawer, Aneta Dydowiczova, Pavel Babica (Brno/CZ)

P 104
Differential effects of pro-inflammatory cytokines on cytotoxic and cholestatic properties of antibiotics
Audrey Burban, Ahmad Sharanek, Andre Guillouzo (Rennes/FR)

P 105
Resveratrol ameliorates acrylamide-induced toxicity in rat cell line IAR20
Lujia Zhang, Li Dong, Liuqing Zhang, Fang Chen (Beijing/CN)

P 106
In vitro evaluation of hepatotoxicity by amiodarone in HepatoPac (micropatterned co-cultured hepatocytes) using liver-specific markers
Zsuzsanna Nerada, Csilla Kegyes, Roelof de Wilde, Zsuzsanna Gáborik (Budaörs/HU)

P 107
Cytotoxicity and DNA damage of a panel of manufactured nanomaterials in rat alveolar epithelial RLE-6TN cells
Fátima Brandão, Sónia Fraga, Carla Costa, Maria João Bessa (Porto/PT), Andrea Haase (Berlin/DE), João Paulo Teixeira (Porto/PT)

P 108
Toxicity of silver ions and silver nanoparticles for organisms of terrestrial ecosystem
Daniela Hlavkova, Barbora Havelkova, Miroslava Beklova (Brno/CZ)

P 109
Cytotoxicity assessment of functional coatings for corrosion protection and controlled degradation of Mg alloys for biomedical application
Sónia Fraga, Susana Silva, Carla Costa (Porto/PT), Isabel Sousa, Cristina Neves (Aveiro/PT), Nico Scharnagl (Geesthacht/DE), Mikhail L. Zheludkevich (Geesthacht/DE, Aveiro/PT), Mário G. Ferreira, João Tedim (Aveiro/PT), João Paulo Teixeira (Porto/PT)

P 110
CYP silenced human hepatocytes for metabolite assessment in a toxic response
Ruoya Li, Belkacem Bouaita, Karine Rondel, David Steen, Flyuza Roshchina, Christophe CHesne (Saint-Grégoire/FR)

P 111
Compound mechanism-of-action, biomarker discovery and preclinical safety studies in samples from six species via DigiWest® multiplex protein profiling
Gerrit Erdmann, Julia Schneider, Anja Briese (Berlin/DE), Yvonne Beiter, Ewa Breitinger, Markus F. Templin (Reutlingen/DE), Christoph Sachse (Berlin/DE)

P 112
Investigation of the potential for Persistent Organic Pollutants (POPs) mixtures to disrupt GLP-1 receptor signalling and hormone secretion and induce cytotoxicity in enteroendocrine pGIP/neo – STC-1 cells
Yuling Xie, Maeve Shannon, Jodie Wilson (Belfast/GB), Hanne Friis Berntsen, Karin Elisabeth Zimmer, Steven Verhaegen, Erik Ropstad (Oslo/NO), Brian Green, Lisa Connolly (Belfast/GB)

P 113
Analysis of the cytotoxic effect of Fe (II)+ in mammalian cell culture
Luqman Khan, Atsushi Higashitani (Sendai/JP)

P 114
Development of new model to evaluate effects on eicosanoid synthesis pathway using invertebrate species
Sangwoo Lee, Mina Cho, Seokjoo Yoon, Woo-Keun Kim (Daejeon/KR)

P 115
An integrated human organ system for evaluating cholestasis
James McKim, Jamin Willoughby, Nicholas Hibbard (Kalamazoo/US)

P 116
Screening of the genotoxic potential of 160 mycotoxins in human cell lines
Marc Audebert, Laure Khoury, Daniel Zalko (Toulouse/FR)

P 117
Adaptations of the comet assay for genotoxicity testing using metabolically active human liver spheroids
Birgitte Lindeman, Edel Lilleaas, Marit Låg, Ann-Karin Olsen, Kristine Gutzkow (Oslo/NO)

P 118
Evaluation of the cytotoxicity of cocaine-caffeine and cocaine-levamisole in precursor cells of the glia
Rene Rocha, Javier Figueroa, Boris Duffau, Ivan Triviño (Santiago/CL)

P 119
Reconstructed 3D model of human small intestine epithelium for prediction of gastrointestinal toxicity and drug absorption
Jan Markus (Bratislava/SK), Helena Kandarova (Bratislava/SK, Ashland/US), Timothy Landry, Seyoum Ayehunie (Ashland/US)

P 120
In vitro model for the prediction of respiratory sensitisation
Aline Chary, Tommaso Serchi, Sebastien Cambier, Elisa Moschini, Servane Contal (Belvaux/LU), Jenny Hennen (Trier/DE), Janine Ezendam (Bilthoven/DE), Brunhilde Blömeke (Trier/DE), Arno Gutleb (Belvaux/LU)

P 121
Human in vitro models for respiratory toxicology – Evaluation of goblet cell hyperplasia, mucus production, and ciliary beating assays
B Gilbert, M. Aragon (Gaithersburg/US), S. Frentzel, J. Hoeng (Neuchatel/CH), S. Ito, S. Ishikawa (Yokohama/JP), J. Budde (Hamburg/DE), A. Maione, P. Hayden (Ashland/US), W. Fields, B. Keyser (Winston-Salem/US), L. Haswell, D. Azzopardi (Southampton/GB), H. Behrsing (Gaithersburg/US)

P 122
Inhalation safety assessment of cosmetic sprays and aerosols
Pierre-Jacques Ferret, Béatrice Bui, Hanna Bondarenkp, Marie-Pierre Gomez-Berrada (Toulouse Cedex/FR)

P 123
Nicotine characterisation of an in vitro inhalation exposure system using conventional and next generation tobacco products – a US/UK interlaboratory comparison
Erin Hill (Gaithersburg/US), Jason Adamson (Southampton/GB), Mario Aragon, Devin Sheehan (Gaithersburg/US), Marianna Gaca (Southampton/GB), Hans Raabe, Holger Behrsing (Gaithersburg/US)

P 124
Implementing alternative approaches for inhalation toxicity testing – Recent success and remaining hurdles
Amy Clippinger (Norfolk/US), David Allen (Research Triangle Park/US), Jon Hotchkiss, Dan Wilson (Midland/US), Annie Jarabek (Research Triangle Park/US)

P 125
Validation of the CULTEX® radial flow system as an in vitro method to screen chemical dusts for their acute inhalation toxicity
Sebastian Hoffmann (Paderborn/DE), Amelie Tsoutsoulopoulos, Katrin Gohlsch (Munich/DE), Niklas Möhle (Hannover/DE), Andreas Breit (Munich/DE), Olaf Krischenowski (Hannover/DE), Dirk Steinritz (Munich/DE), Michaela Aufderheide (Hannover/DE)

P 126
GARDair – A novel assay for assessment of chemical respiratory sensitisers based on a genomic platform
Henrik Johansson, Andy Forreryd, Robin Gradin, Angelica Johansson, Olivia Larne, Emil Aaltonen, Anders Jerre, Carl Borrebaek, Malin Lindstedt (Lund/SE)

P 127
New human cell models mimicking various respiratory regions for nanoparticle toxicology – Which is the more suitable?
Lars Leibrock, Sandra Wagener, Tanja Vogt, Peter Laux, Andreas Luch (Berlin/DE)

P 128
In vitro toxicological evaluation of emissions from catalytic oxidation of industrial VOCs by air/liquid interface (ALI) exposure system in repeated exposure mode
Clémence Meausoone, Rebecca Khawaja, Gauthier Tremolet, Stéphane Siffert, Renaud Cousin, Fabrice Cazier, Sylvain Billet, Dominique Courcot, Yann Landkocz (Dunkerque/FR)

P 129
Popcorn lung – Diacetyl exposure of primary human bronchial epithelial cells using a relevant air-liquid setup
Detlef Ritter, Jan Knebel (Hannover/DE), Jan J.W.A. Boei, Harry Vrieling (Leiden/NL), Britta Kühne (Hannover/DE), Pieter S. Hiemstra (Leiden/NL), Tanja Hansen (Hannover/DE)

P 130
Use of QSAR models to investigate potential inhalation acute toxicity in liquids used in electronic cigarettes
Daniele Zarini (Milan/IT), Alessandro Sangion, Enrico Caruso (Varese/IT), Silvia Sterpone, Sara Zucchi (Milan/IT), Alessandro Orro (Segrate/IT), Emanuele Ferri (Milan/IT), Ester Papa (Varese/IT)

P 131
Are clinically relevant toxicities inducible and analysable in the 3-D human in vitro airway model EpiAirway®-FT? – A proof of concept study
Darren Kidd, Amber Woodhams, Andrew Daunt, Andrew Postoyalko (Harrogate/GB)

P 132
In vitro toxicogenomic and proteomic assessment of e-cigarette aerosol compared to cigarette smoke
Stela Bozhilova, Ivan Verrastro, Linsey Haswell, Sarah Corke, Andrew Baxter, Samuel Loveridge, Emmanuel Minet, Damien Breheny, Marianna Gaca (Southampton/GB)

P 133
An approach to testing undiluted e-cigarette aerosol in vitro using 3D human airway epithelium
Linsey Haswell, Emma Bishop, Ioanna Vardakou, Jason Adamson, Sandra Costigan, David Thorne, Marianna Gaca (Southampton/GB)

P 134
Inter-laboratory performance of mucin ELLA, CBF and TEER measurements on bronchial MucilAir cultures
Linsey Haswell, Sarah Corke (Southampton/GB), Samuel Constant, Bernadette Boda (Geneva/CH), Marie Sgandurra, Stefan Frentzel, Karsta Luettich, Patrice Leroy, Julia Hoeng (Neuchatel/CH), Marianna Gaca (Southampton/GB)

P 135
Evaluation of in vitro models of the rat and human airway epithelium for assessment of acute airway toxicity
Anna Maione, George R Jackson (Ashland/US), Joanne Vinall, Hazel Simpson, Eleanor Storey (Edinburgh/GB), Michelle Debatis, Mitchell Klausner (Ashland/US), Helena Kandarova (Bratislava/SK), Clive Roper (Edinburgh/GB), Patrick Hayden (Ashland/US)

P 136
Carbendazim induces death in alveolar epithelium cell
Bruna, T. Tollstadius, Artur Christian Garcia Silva, Bruna Cristiane Oliveira Pedralli, Marize campos Valadares (Goiânia/BR)

P 137
Pairwise toxicity evaluation of ceramic nanoparticles exposure in human alveolar epithelial A549 cells at submerged and air-liquid cultures
Maria João Bessa, Sónia Fraga, Fátima Brandão (Porto/PT), Paul Fokkens, John Boere, Daan Leseman (Utrecht/NL), Apostolos Salmatonidis, Mar Viana (Barcelona/ES), Flemming Cassee (Utrecht/NL), João Paulo Teixeira (Porto/PT)

P 138
A novel approach for the screening of e-cigarette aerosols using an Ames whole aerosol assay
David Thorne (Southampton/GB), Michael Hollings, Adam Seymour (Harrogate/GB), Ian Crooks, Clive Meredith, Marianna Gaca (Southampton/GB)

P 139
Natural hair colouring cosmetics adulterated with PPD showed a higher potential to trigger allergic contact dermatitis in comparison to PPD alone
Renato Ivan de Ávila, Danillo Veloso, Gabriel Teixeira (Goiânia/BR), Tim Lindbergh, Malin Lindstedt (Lund/SE), Thaisângela Rodrigues, Simone Fonseca, Eliana Lima, Marize Valadares (Goiânia/BR)

P 140
Analysis of global proteome changes in THP-1 cells after treatment with contact sensitisers
Tessa Höper, Murat Eravci, Heike Stephanowitz, Eberhard Krause, Andrea Haase, Andreas Luch (Berlin/DE)

P 141
Coculture of THP-1 cells with 3D reconstructed human epidermis for the assessment of skin sensitising potential of chemicals
Mario T Schellenberger, Udo Bock, Jennifer Hennen (Trier/DE), Florian Groeber-Becker, Heike Walles (Würzburg/DE), Brunhilde Blömeke (Trier/DE)

P 142
Application of an integrated assessment and testing approach for skin sensitisation within a ranking system for epoxy resin compounds
Karin Heine (Freiburg/DE), Johannes Geier (Göttingen/DE), Fritz Kalberlah (Freiburg/DE)

P 143
A new prediction model for distinguishing skin sensitisers based on IL-18 release from reconstructed epidermis – Enhancing the assessment of a key event in the skin sensitisation adverse outcome pathway
Eric Andres, Mouctar Barry, Alexander Hundt, Christophe Dini (Romainville/FR), Pierre-Jacques Ferret (Toulouse/FR)

P 144
Successful proof of concept of a micronucleus genotoxicity assay on reconstructed epidermis exhibiting intrinsic metabolic activity
Rola Barcham (Romainville/FR), Nicolas Orsini (Biot/FR), Eric Andres, Alexander Hundt, Christophe Dini (Romainville/FR), Anne-Pascale Luzy (Biot/FR)

P 145
Human serum lot comparison for the animal-product-free adaptation of the h-CLAT method
Alex Edwards, Lottie Roscoe, Chris Longmore, Fiona Bailey, Bushra Sim, Carol Treasure (Daresbury/GB)

P 146
Investigation into the effects of metabolism on the cytotoxicity of a subset of cosmetically-relevant compounds using an animal-product-free assay
Fiona Bailey, Alex Edwards, Lottie Roscoe, Chris Longmore, Bushra Sim, Chris Taylor, Nathalie Belot, Carol Treasure (Daresbury/GB)

P 148
The use of High Content Screening (HCS) in human primary lung cells to assess e-liquids
Liam Simms, Lukasz Czekala, Matthew Stevenson (Bristol/GB), Tanvir Walele (Amsterdam/NL)

P 149
Skin sensitisation testing and proteomic analysis of the effects of glyphosate, the co-formulant POAE and herbicide products on a dendritic cell model
M Lindberg, T Lindberg (Lund/SE), R I de Ávila (Goiânia/BR), K S Zeller, F Levander (Lund/SE), D Eriksson, A Chawade (Alnarp/SE)

P 150
Regulation of distinct miRNAs and RNA transcripts by structurally similar rubber sensitisers in a dendritic cell model
K S Zeller, T Lindberg, A Forreryd (Lund/SE), O Bergendorff (Malmö/SE), R Søkilde, C Rovira, M Lindberg (Lund/SE)

P 151
Evaluation of antioxidant activity and phototoxic potential of quercetin-loaded solid lipid nanoparticles (SLN) using in vitro models
Camila Martins Kawakami, Renata Spagolla Napoleão Tavares, Thais Priscilla Pivetta, Priscyla Daniely Marcato Gaspari (Ribeirão Preto/BR), Silvya Stuchi Maria-Engler (São Paulo/BR), Lorena Rigo Gaspar (Ribeirão Preto/BR)

P 152
Prediction of photosafety and antioxidant properties of fucoxanthin by using in vitro methods
Renata Spagolla Napoleão Tavares, Camila Martins Kawakami (Ribeirão Preto/BR), Pio Colepicolo Neto (São Paulo/BR), Hosana Maria Debonsi, Lorena Rigo Gaspar Cordeiro (RIbeirão Preto/BR)

P 153
High accuracy of the short time exposure test for non-diluted and diluted substances
Takuo Yuki, Takayuki Abo, Yutaka Takahashi, Hitoshi Sakaguchi (Tochigi/JP)

P 154
The EyeIRR-IS assay – Development of an in vitro method using SkinEthic HCE model for liquid chemical eye irritation sub-categorisation
Herve Groux (Grasse, Aulnay/bois/FR), Francoise Cottrez (Grasse/FR), Nathalie Alépée, Virginie Leblanc (Aulnay/bois/FR)

P 155
Using the SENS-IS assay to assess skin sensitisation hazard of commercial cosmetics finished products
Herve Groux, Francoise Cottrez, Elodie Boitel (Grasse/FR)

P 156
Assessment of pre/prohaptens and surfactants using the SENS-IS assay to measure skin sensitisation potency
Herve Groux, Francoise Cottrez, Elodie Boitel (Grasse/FR)

P 157
In vitro assessment of medical device extracts potential to produce skin sensitisation
Herve Groux, Francoise Cottrez (Grasse/FR), Christian Pellevoisin (Lyon/FR), Kelly Coleman (Minneapolis/US)

P 158
Phototoxicity potential of combinations of insect repelents and UV filters
Carolina Gomes Benevenuto, Camila Martins Kawakami, Karen Cristina Rangel, Renata Spagolla Napoleão Tavares (Ribeirão Preto/BR), Silvya S. Maria-Engler (São Paulo/BR), Lorena Gaspar (Ribeirão Preto/BR)

P 159
A mechanistic reinterpretation of the AOP for skin sensitisation
David Roberts (Wirral/GB)

P 160
Tattooed reconstructed human skin for the in vitro assessment of pigment-associated genotoxicity
Henrik Hering, Ines Schreiver, Christian Zoschke, Markus Kühn, Günther Weindl, Andreas Luch (Berlin/DE)

P 161
Determination of contact sensitisation potential of 20 chemicals using in vitro reconstructed normal human epidermal model EpiDerm: – Impact of the modality of application
Silvia Letasiova (Bratislava/SK), Emanuela Corsini, Valentina Galbiati (Milan/IT), Helena Kandarova (Bratislava/SK)

P 162
Inter-and intra-laboratory reproducibility of the in vitro photo-toxicity test using 3D reconstructed human epidermis model EpiDerm
Helena Kandarova, Alžbeta Líšková (Bratislava/SK), Bushra Sim, Fiona Bailey, Carol Treasure (Daresbury, Cheshire/GB), Dagmar Jírová, Kristína Kejlová (Prague/CZ), Silvia Letašiová (Bratislava/SK)

P 164
In vitro comparative cytotoxicity study of aminated polystyrene, zinc oxide and silver nanoparticles on a cervical cancer cell line
Akash Sharma, Alan Casey (Dublin/IE)

P 165
Comparison of 2D and 3D cultures of primary hepatocytes on hepatocellular functions and hepatotoxicity
Heiko Dinter (Biberach/DE), Anett Ullrich, Dieter Runge (Schwerin/DE)

P 166
Mass spectrometric studies on the in vitro metabolism of various pyrrolizidine alkaloids
Ina Geburek, Franziska Kolrep, Angelika Preiß-Weigert, Monika Lahrssen-Wiederholt (Berlin/DE), Dieter Schrenk (Kaiserslautern/DE), Anja These (Berlin/DE)

P 167
Toxicity of diuron metabolites in human cell
Ali Mohammed, Marjo Huovinen, Kirsi Vähäkangas (Kuopio/FI)

P 168
Human isolated small bowel specimens to assess potential new therapeutics for organophosphate poisoning
Katharina Marquart, Olga Prokopchuk, Franz Worek, Horst Thiermann, Marc E. Martignoni, Timo Wille (Munich/DE)

P 169
Co-culture systems may enable to detect the effects of bisphenols on certain signaling pathways
Florian Padberg, Patrick Tarnow, Andreas Luch, Sebastian Zellmer (Berlin/DE)

P 170
Development of predictive in vitro animal proximal tubule models for drug transporter and nephrotoxicity studies
Git Chung, Lyle Armstrong, Mike Nicholds, Colin Brown (Newcastle upon Tyne/GB)

P 171
Butyrate alters expression and activities of xenobiotic metabolising enzymes in colon epithelial cell models
Ondrej Zapletal, Jirina Prochazkova, Vit Dubec, Jirina Hofmanova, Alois Kozubik, Jan Vondracek (Brno/CZ)

P 172
Inhibition of P300/CBP reduces CYP1A1 induction in cancer cell models derived from liver or colon epithelium
Martin Krkoska, Jana Svobodová, Ondrej Zapletal, Martina Karasova, Alena Hyrslova Vaculova, Jan Vondracek (Brno/CZ)

P 173
Novel collagen based scaffold which allows shipment and direct metabolic studies of human hepatocytes over 10 days
Marc Ruoß, Sabrina Ehnert (Tübingen/DE), Frank Schügner, Leon Olde Damink (Herzogenrath/DE), Serene Lee, Liming Ge (Planegg/Martinsried, Munich/DE), Andreas Nussler (Tübingen/DE)

P 174
Real-time monitoring of oxygen consumption of hepatocytes in a microbioreactor
Katja Uhlig, Christian Gehre (Potsdam/DE), Sarah Kammerer, Jan-Heiner Küpper (Senftenberg/DE), Dominic Coleman, Gerhard Paul Püschel, Claus Duschl (Potsdam/DE)

P 175
Evaluation of models to estimate the distribution kinetics of test chemicals in vitro
Susana Proença (Utrecht/NL), Andreas Schepky (Hamburg/DE), Nicola J. Hewitt (Brussels/BE), Beate Nicol (Sharnbrook/GB), Martina Klaric, Bertrand Desprez (Brussels/BE), Gladys Ouedraogo (Saint-Ouen/FR), Nynke Kramer (Utrecht/NL)

P 176
PBPK modeling of zebrafish embryo for reprotoxicity assessment of valproic acid and some of its analogs
Segolene Simeon (Verneuil-en-Halatte/FR), Katharina Brotzmann (Heidelberg/DE), Ciaran Fisher, Iain Gardner (Sheffield/GB), Thomas Braunbeck (Heidelberg/DE), Richard Maclennan (Cheshire/GB), Frederic Bois (Verneuil-en-Halatte/FR)

P 177
Steroids and pain killers – Influences of NSAIDs on human steroid metabolism
Anna Stoll (Berlin/DE), Xavier de la Torre, Francesco Botrè (Rome/IT), Maria Kristina Parr (Berlin/DE)

P 178
An advanced multi-cellular and dynamic flow model of the human alveolar airway to study the impact of inhaled particulate
Joana Moura (Swansea/GB), Iris Muller (Colworth/GB), Shareen Doak, Martin Clift (Swansea/GB)

P 179
In vitro to in vivo extrapolation – Importance of the presence of protein in in vitro models for prediction of in vivo effects
Kenneth Brouwer, Jonathan Jackson (Durham/US)

P 180
Inclusion of gastro-intestinal pH conditions in nanoparticle toxicity testing – Results of an in vivo-in vitro comparison
Mathias Busch, Angela Kämpfer (Düsseldorf/DE), Burkhard Stahlmecke, Bryan Hellack (Duisburg/DE), Catrin Albrecht, Roel Schins (Düsseldorf/DE)

P 181
Rapid and accurate high-throughput in vitro prediction of nephrotoxicity in humans with ProxTox-HTS
Csilla Kegyes (Budaörs/HU), Daniele Zink (The Nanos/SG)

P 182
Real-time assays to monitor cell health for in vitro toxicokinetic models
Terry Riss (Madison/US)

P 183
Development of highly sensitive and specific in vitro renal solute carrier (SLC) uptake cell models using genetically modified human renal proximal tubule epithelial cells for renal drug transporter interaction studies
Johanna Lier (Wesel/DE), Chaozhong Zou, Luis Romero, Elizabeth Turner (Gaithersburg/US), Kevin Huang, Alice Gibson (Columbus/US), Penney McWilliams-Koeppen, Brian Chase (Gaithersburg/US)

P 184
Analytical measure of intracellular biodistribution of chemicals as a tool to understand toxicological effects
Stefano Lorenzetti (Roma/IT), Antonella Smeriglio (Roma, Messina/IT), Daniele Marcoccia (Roma/IT), Domenico Trombetta (Messina/IT), Alberto Mantovani (Roma/IT), Costanza Rovida (Konstanz/DE)

P 185
In vitro cytotoxic effect of real life exposure of persistent organic pollutants (POP"s) on human kidney cells (A498) using emerging high content analysis technology
Mazia Amber (Belfast/GB), Hanne Fris Berntsen, Karin Elisabeth Zimmer, Steven Verhaegen, Erik Ropstad (Oslo/NO), Maeve Shannon, Lisa Connolly (Belfast/GB)

P 187
Applicability in vitro bacterial model for the equivalence assessment of pesticide technical materials
Valerii Rakitskii, Nataliya Ilyushina, Olga Egorova (Mytishchi/RU)

P 188
Validation of a non-animal vaginal irritation method admitted as nonclinical assessment model (NAM) in the incubator phase of the United States Food and Drug Administration (US FDA) Medical Devices Development Tool (MDDT)
E. Hill (Gaithersburg/US), J. Brown (London/GB), G.-E. Costin, R. Curren (Gaithersburg/US), J. Sirois (Washington, DC/US), L. Bernhofer, A. Ghassemi (Princeton/US), P. Rao (White Plains/US), K. Acuff, K. Blieszner (Cincinnati/US), L. Burns (Parsippany/US), P. Clay (Hull/GB), S. Berry, C. Platt (Carlsbad/US)

P 189
The metabolic capacity of cell-based assays and incorporation of metabolism into in vitro assays for endocrine disruption
Camilla Taxvig, Anne Marie Vinggaard, Terje Svingen (Kgs. Lyngby/DK)

P 190
A novel approach for the screening of e-cigarette aerosols using an ames whole aerosol assay
David Thorne (Southampton/GB), Michael Hollings, Adam Seymour (Harrogate/GB), Ourania Komini, Ian Crooks, Clive Meredith, Marianna Gaca (Southampton/GB)

P 192
Perspectives of in vitro methods application for classification of pesticides for skin corrosion/irritation and eye irritation in Ukraine
Nataliia Bubalo, Serhii Kolesnyk, Mykola Prodanchuk, Petro Zhminko (Kyiv/UA)

P 193
Good in vitro method practices (GIVIMP) as a response to a more general "crisis in reproducibility" in scientific data generation
Sandra Coecke, Gerard Bowe (Ispra/IT), Patience Browne (Paris/FR)

P 194
How the good in vitro method practices guidance document can help implement new toxicological approaches – A case study in China
Sandra Coecke, Gerard Bowe, Maurice Whelan (Ispra/IT), Barbara Birk, Susanne Kolle, Bennard van Ravenzwaay, Robert Landsiedel (Ludwigshafen/DE), Amanda Ulrey, Erin Hill (Gaithersburg/US)

P 195
Bioassays to assess unpredictable substances from drinking water contact materials
Ralf Junek, Tamara Grummt, Thomas Rapp, Ramona Schuster (Bad Elster/DE), Alexander Link, Roland Widler, Marcus Bohl, Christian Ragoss (Köln/DE)

P 196
The european consensus platform for 3R alternatives to animal experimentation (ecopa)
Costanza Rovida (Konstanz/DE), Luisa Bastos (Brussels/DE), Philippe Hubert (Verneuil en Halatte/FR), Tuula Heinonen (Tampere/FI)

P 197
Development and validation of the EpiDerm in vitro skin irritation protocol for the assessment of medical devices extracts
Helena Kandarova, Silvia Letašiová (Bratislava/SK), Michael Bachelor (Ashland, MA/US), Tatiana Milasova (Bratislava/SK)

P 198
Study of the inflammasome NLRP3 and the role of NLRP12 protein after exposure to allergens of different potency
Marica Pisapia, C. Lossani, T. Simone, A. Papale, V. Galbiati, E. Corsini (Milan/IT)

P 199
Biocompatibility test regarding ISO 10993 (Part 5 Cytotoxicity) of novel developed colostomy bags from deproteinized natural rubber latex and LLDPE resins
Kwunchit Oungbho, Worrawit Wanichsuwan, Ekwipoo Kalkornsurapranee (Songkhla/TH)

P 201
A 10-year study on clinical prognostic factors in food-borne botulism
Sara Saeidi Shahri, Bita Dadpour, Lida Jarahi, Anahita Alizadeh Ghamsari, Mehdi Jabbari Nooghabi (Mashhad/IR)

P 202
The citrus flavonone hesperetin attenuates the nuclear translocation of Aryl hydrocarbon Receptor
Lai K. Leung (Shatin/HK)

P 203
Nutritional modulation of the toxicity of environmental pollutants and implications in translational toxicology
Bernhard Hennig, Michael Petriello (Lexington/US)

P 204
Neurospheroids – Refining iPSC-based 3D neural cell models to address brain microenvironment related diseases
Ana Paula Terrasso, Daniel Simão, Marta M. Silva, Francisca Arez (Oeiras/PT), Neus Bayó-Puxan (Barcelona/ES, Montpellier/FR), Marcos F. Sousa, Patrícia Gomes-Alves (Oeiras/PT), Nuno Raimundo (Göttingen/DE), Eric J. Kremer (Montpellier/FR), Paula M. Alves, Catarina Brito (Oeiras/PT)

P 205
In vitro cell mechanical stretching – A promising method to replace skin expansion animal models
Shi Fu, Jiansong Wang, Haifeng Wang, Ting Luan, Yinglong Huang (Kunming/CN)

P 206
Toxicity of natural uranium on an osteocyte murine cell line
Lucile Hurault, Valérie Pierrefite-Carle, Sabine Santucci-Darmanin, Sophie Pagnotta, Gaëlle Creff, Christophe Den Auwer, Georges F. Carle (Nice/FR)

P 207
New criteria for classification of materials hazardous in bulk
Nicole Mazurek, Renate Krätke (Berlin/DE)

P 208
Preliminary assessment of defined serum-free medium in the h-CLAT method
Christopher Longmore, Alexander Edwards, Jainah Halliday, Carol Treasure (Daresbury/GB)

P 209
Evaluation of data from previously conducted in vivo animal studies against the results of the threshold calculation for agrochemical formulations
William Masinja (Liverpool/GB)

P 210
Photochemoprotective potencial and toxicity assessment of the Curdiea racovitzae extract
Karen Cristina Rangel, Ana Elisa Caleiro Seixas Azzolini, Hosana Maria Debonsi (Ribeirão Preto/BR), Pio Colepicolo (São Paulo/BR), Lorena Rigo Gaspar (Ribeirão Preto/BR)

P 211
Functionally immortalised cellular test systems for bioassay development and in vitro toxicology testing
Tom Wahlicht, Theresa Truschel, Kristina Nehlsen, Roland Schucht, Dagmar Wirth, Tobias May (Braunschweig/DE)

P 212
Skin sensitisation, cancer-specific cytotoxicity, and hepatotoxicity as major effects of novel purine-analog phosphonates
Mathias Dunkel, Christian Zoschke (Berlin/DE), Robert Landsiedel (Frankenthal/DE), Vivian Kral, Nan Zhang (Berlin/DE), Britta Waering (Frankenthal/DE), Hans-Dieter Höltje (Düsseldorf/DE), Robert Preissner, Monika Schäfer-Korting (Berlin/DE)

P 214
Next generation risk assessment approach for skin allergy – Case study-driven evaluation
Maria Baltazar, Gavin Maxwell, Nicola Gilmour, Joe Reynolds, Evita Vandenbossche, James Butcher, Richard Cubberley, Cameron Mackay, Ruth Pendlington (Bedford/GB)

P 215
Regarding the references for reference chemicals of alternative methods
Susanne Kolle (Ludwigshafen/DE), E. Hill, H. Raabe (Gaithersburg/US), R. Landsiedel (Ludwigshafen/DE), R. Curren (Gaithersburg/US)

P 216
Suitability of human induced pluripotent stem cells in hepatic toxicity assessment using perfused 3D bioreactors
Nora Freyer, Selina Greuel (Berlin/DE), Georg Damm, Daniel Seehofer (Leipzig/DE), Frank Jacobs (Beerse/BE), Katrin Zeilinger, Fanny Knöspel (Berlin/DE)

P 217
Assay ready frozen THP-1 cells can be used like a reagent in a human Cell Line Activation Test (h-CLAT) to measure the skin sensitizing potential of chemicals
Vladimir Mazurov, J. Walbech, A. Loa, O. Wehmeier (Hamburg,/DE)

P 218
Crowd-verified causal network models for xenobiotic metabolism in the liver – Methods for 3R
Justyna Szostak, Stephanie Boue, Marja Talikka, Giuseppe Lo Sasso, Sylvain Gubian, Manuel C. Peitsch, Julia Hoeng (Neuchâtel/CH)

P 219
Coupling of yeast and bacterial based bioreporters with high performance thin layer chromatography
Carolin Riegraf (Koblenz, Aachen/DE), Georg Reifferscheid (Koblenz/DE), Liat Moscovici, Dror Shakibai, Shimshon Belkin, Nidaa Abu Rmalah (Jerusalem/IL), Sebastian Buchinger (Koblenz/DE)

P 220
Impact on the human HepG2 cell metabolome of exposure to a mixture of 2 persistent organic pollutants
Cédric Caradeuc, Béatrice Le-Grand, Aude Catalayud, Adama Ka, Martine Aggerbeck, Gildas Bertho, Etienne Blanc (Paris/FR)

P 221
In vitro cytotoxicity test on MC3T3-E1 cells of herbal extracts in longevity herbal preparation
Kwunchit Oungbho, Sawanee Kraithep, Supinya Tewtrakul (Songkhla/TH)

P 222
Histological analysis of buccal and small-airway organotypic cultures exposed to cigarette smoke and electronic cigarette aerosol
Keyur Trivedi, Anita R. Iskandar, Filippo Zanetti, Laurent Neau, Francesca Caravello, Petros Kanellos, Stefan Frentzel, Julia Hoeng (Neuchâtel/CH)

P 223
CYP1A1-mCherry reporter hiPSC based high-throughput chemical screening for the identification of aryl hydrocarbon receptor ligands
Ji-Woo Kim, Hye-Min Kim, Hang-Suk Chun, Seokjoo Yoon, Han-Jin Park (Daejeon/KR)

P 224
Towards a microencapsulated 3D hiPSC-derived in vitro cardiac microtissue for recapitulation of human heart microenvironment features
Bernardo Abecasis, Pedro Costa, Henrique Almeida (Oeiras/PT), Susana Rosa, Pedro Gouveia (Coimbra/PT), Patrícia Gomes-Alves (Oeiras/PT), Lino Ferreira (Coimbra/PT), Margarida Serra, Paula M. Alves (Oeiras/PT)

P 225
Investigating the mode of action for DNA damage by flavors in ENDS using in vitro and in silico screening
Luis G. Valerio, Jr. (Silver Spring, MD/US), Luis G. Valerio, Jr., Pei-Hsuan Hung, Mamata De, Matthew Savidge (Silver Spring, MD/US)

P 226
Protocol comparison for deriving BBB-models from iPSCs for use in toxicological assessment of chemicals in the light of the in3-project
Sara Wellens, Lucie Dehouck, Marjolein Heymans, Fabien Gosselet, Maxime Culot (Lens/FR)

P 227
Effects of an emerging Fusarium mycotoxin on hepatocytes
Eriksen Gunnar Sundstøl, Bernd Thiede, Anita Solhaug (Oslo/NO)

P 228
The impact of the MeHgCl on the regulation of mitochondrial biogenesis in the hiPS cells cultured in the different oxygen conditions
Justyna Augustyniak, Jacek Lenart, Gabriela Lipka, Leonora Buzanska (Warsaw/PL)

P 229
Numerical characterisation of aerosol exposure systems – an analytical model to predict dose deposition
F. Lucci (Neuchâtel/CH), Arkadiusz Kuczaj (Neuchâtel/CH, Enschede/NL)

P 230
Evaluation of cytotoxicity of Clostridium perfringens epsilon toxin on MDCK cell and its use in Entrotoxemia vaccine potency test
Anahita Emadi (Tehran/IR)

P 231
EpiIntestinal on a BioChip
Joachim Wiest (Kronburg/DE), Ch. Schmidt (Kronburg, Munich/DE), J. Markus, H. Kandarova (Bratislava/SK)
P 232Co-culture of primary hepatic stellate cells and hepatocytes in modeling of liver fibrosis in 2D and 3D spheroids
Rafal P. Witek, Sujoy Lahiri, Bina Khaniya, Michael P. Connolly, Debra L. Johnson, Mark Kennedy, Deborah K. Tieberg, Gillian Claire Wallace (Paisley/GB)
P 233Characterisation of 3D cultured primary human hepatocytes and HepaRG for DMSO-free preclinical toxicity testing
Camille Savary, Kathleen Jarnouen, Stéphanie Molez, Pierre-Jean Ferron, Nathalie Maubon, Anne Corlu (Rennes/FR)

Postconference workshop, 18-19 October 2018


The postconference workshop will combine theoretical introductions as well as practical trainings of 3 topics relevant to the fields of in vitro and in silico toxicology.

In vitro skin irritation testing
(Helena Kandárová and Silvia Letasiova, MatTek In Vitro Life Science Laboratories, Bratislava, Slovak Republic)

Participants will be given an overview of existing models and protocols for assessment of skin irritation of chemicals, cosmetics and medical devices, including their validation status and regulatory acceptance. The practical training will be focused on the handling of the reconstructed epidermis model EpiDerm™ as well as on different application techniques required in the different protocols. Each participant will get the opportunity to test a solid, a liquid and a semi-solid substance on the reconstructed epidermis.

Organ-on-a-chip modeling 
(Isabel Rütschle, Daniel Faust and Reyk Horland, TissUse GmbH, Berlin, Germany)

Participants will be given an overview of the organ-on-a-chip field, including examples of established organ models and organ combinations for use in substance testing. Aspects of qualification and validation will also be discussed. The practical training will be focused on the use and handling of multiorgan-chip devices. By setting up a skin-liver co-culture model, participants will acquire insight into the preparation of chips, cells and organ models, the experimental set-up and loading of the chip, substance exposure, sampling and in-process analytics, and endpoint controls.

In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
(Nynke Kramer, Utrecht University, The Netherlands)

Participants will be given an introduction to the mathematical basis and applications of physiologically based kinetic (PBK) modeling and quantitative in vitro-in vivo extrapolation (QIVIVE). During the practical training, participants will be able to apply this knowledge by constructing their own PBK model of toluene in rats and humans, and applying it to extrapolate in vitro neurotoxic effect concentrations to dose-equivalent levels of human exposure.

Thursday, 18 October 2018: Theoretical introductions (Main auditorium)
14.30–16.30Skin irritation testing and organ-on-a-chip models
16.30–16.45Break
16.45–17.30In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
Friday, 19 October 2018: Demonstrations and hands-on training (Nizza room)
8.30–10.30Skin irritation testing and organ-on-a-chip models 
10.30–10.45Break
10.45–12.30Skin irritation testing and organ-on-a-chip models
12.30–13.00Lunch
13.00–15.00In silico physiologically based kinetic modeling and quantitative in vitro-in vivo extrapolation
15.00Wrap-up, handing over of training certificates with photographing, adjourn

Participants can register for attending either only the theoretical introductions (Thursday 18 October 2018) or both the theoretical introductions and the practical training (Thursday 18 October and Friday 19 October 2018), at a cost of 40€ or 85€, respectively. The registration fee includes a booklet, coffee breaks and lunch (only served on Friday 19 October 2018). While there is no limitation in the number of participants attending the theoretical introductions, only 25 persons can register for both the theoretical introductions and the practical training. Registration only for the practical training is not possible. The language of the postconference workshop will be English with no simultaneous translation to other languages. Persons attending the practical training need to bring their own portable computer for the in silico training.